Probe:	Cx36 probe3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Moderate		2a	Expressed in the most anterior territory of the brain.
TS15: optic cup	Present		2a
TS15: optic stalk	Present		2a

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Moderate		2b	Expressed in the most anterior territory of the brain.
TS15: optic cup	Present		2b
TS15: optic stalk	Present		2b

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		2c	Expressed in the most anterior territory of the brain. Expressed in the proliferative neuroepithelium.
TS17: midbrain	Present	Regionally restricted	2c	Expressed in the most anterior territory of the brain. The caudal margin extends posteriorly to the pretectum area. Expressed in the proliferative neuroepithelium.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		2d	Expressed in the most anterior territory of the brain. Expressed in the proliferative neuroepithelium.
TS17: midbrain	Present	Regionally restricted	2d	Expressed in the most anterior territory of the brain. The caudal margin extends posteriorly to the pretectum area. Expressed in the proliferative neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: midbrain-hindbrain junction	Strong	Regionally restricted	2e	Expressed as two strong signals in the ventral part.
TS20: thalamus	Present	Regionally restricted	2e	Expressed in a stripe that is localized at the boundary between the dorsal and ventral region (zona limitans intrathalamica).

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Strong	Regionally restricted	2f	Expressed in a strong stripe that is localized at the boundary between the dorsal and ventral region. Expressed in the dorsal telencephalon but the density is much weaker than in previous stages.
TS20: thalamus	Present	Regionally restricted	2f	Expressed in a stripe that is localized at the boundary between the dorsal and ventral region (zona limitans intrathalamica).

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	2g	Expressed in a stripe that is localized at the boundary between the dorsal and ventral region (zona limitans intrathalamica).

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2m	Expressed in the most ventrolateral region of the dorsoventral boundary.
TS21: midbrain roof plate	Present	Regionally restricted	2m	Expressed in the region that will give rise to the superior colliculus.
TS21: cerebellum	Present		2m	Rhombic lip.

Structure	Level	Pattern	Image	Note
TS21: midbrain roof plate	Present	Regionally restricted	2n	Expressed in the region that will give rise to the superior colliculus.
TS21: cerebellum	Present		2n	Rhombic lip.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Strong	Regionally restricted	3e	Expressed in lateral and ventral stripes.

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Strong	Regionally restricted	3f	Expressed in lateral and ventral stripes.

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Strong	Regionally restricted	3g	Expressed in lateral and ventral stripes.

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Strong	Regionally restricted	3h	Expressed in lateral and ventral stripes.

Structure	Level	Pattern	Image	Note
TS13: future brain	Absent

Structure	Level	Pattern	Image	Note
TS20: spinal ganglion	Weak	Regionally restricted		Along the trunk.
TS20: sympathetic ganglion	Weak	Regionally restricted		Along the trunk.
TS20: spinal cord	Weak	Regionally restricted		Expressed as longitudinal stripes.