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Wnt10btm1(cre/ERT2)Amc
Targeted Allele Detail
Summary
Symbol: Wnt10btm1(cre/ERT2)Amc
Name: wingless-type MMTV integration site family, member 10B; targeted mutation 1, Andrew P McMahon
MGI ID: MGI:5660815
Synonyms: Wnt10b-G2aCE, Wnt10btm1(GFP,cre/ERT2)Amc
Gene: Wnt10b  Location: Chr15:98668593-98676031 bp, - strand  Genetic Position: Chr15, 54.65 cM
Alliance: Wnt10btm1(cre/ERT2)Amc page
Mutation
origin
Mutant Cell Line:  EPD0314_2_A09
Germline Transmission:  Earliest citation of germline transmission: J:159099
Parent Cell Line:  JM8A1.N3 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Mutation
description
Allele Type:    Targeted (Inducible, Null/knockout, RMCE-ready, Recombinase, Reporter)
Inducer:    tamoxifen
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Wnt10btm1(cre/ERT2)Amc expression driven by 1 gene
 
Mutation details: An exchange vector was designed to be inserted downstream of exon 1 of the gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5 to 3) an FRT site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein sequence, a viral T2A oligopeptide, and a Cre-ERT2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand-binding domain). A rox-flanked puromycin resistance cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Wnt10b (mouse)
Summary of all recombinase alleles driven by Wnt10b.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Wnt10b Mutation:  18 strains or lines available
Notes
The exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-derived JM8A1.N3 knockout first reporter embryonic stem (ES) cells obtained from the Knockout Mouse Project (KOMP) consortium containing clone EPD0314_2_A09. Recombined ES cells lack the neo cassette and are resistant to puromycin.
References
Original:  J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010;
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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04/16/2024
MGI 6.23 		The Jackson Laboratory