Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149253
Kulkarni RM, et al., NFATc1 regulates lymphatic endothelial development. Mech Dev. 2009 May-Jun;126(5-6):350-65
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5660396
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Gene symbol:
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Nfatc1
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Gene name:
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nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1
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Antibody:
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anti-NFATc1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Note: Double labeled: green - Nfatc1; red - Prox1 (assay MGI:5660553).
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nfatc1; red - Prox1 (assay MGI:5660553).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cardinal vein |
Present |
Regionally restricted |
1A
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Expressed in the cardinal vein endothelial cells and lymphatic endothelial cells sprouting from the cardinal vein. Expressed in nuclei, as shown in inset. Nfatc1 and Prox1 were colocalized only in the regions of active lymphatic sprouting.
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Specimen
1B/D:
embryonic day 12.5
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
1B/D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cardinal vein |
Present |
Regionally restricted |
1D
,
1B
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Expressed in endothelial cells. Expressed in nuclei. Coexpressed with Prox1, but not Flt4.
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| TS20: lymphatic system |
Present |
Uniform |
1B
,
1D
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Uniform bilateral expression in jugular lymph sacs endothelial cells. Expressed in nuclei. Coexpressed with Prox1 and Flt4.
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| TS20: venous system |
Present |
Single cells |
1B
,
1D
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Expressed in a few jugular vein endothelial cells, which are Prox1- and Flt4-negative. Expressed in nuclei.
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Specimen
1E:
embryonic day 12.5
(more )
Note: Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
Present |
Uniform |
1E
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Expressed in jugular lymph sacs endothelial cells. Expressed in nuclei. Coexpressed with Prox1 and Pdpn.
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| TS20: cardinal vein |
Present |
Regionally restricted |
1E
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Expressed in endothelial cells. Expressed in nuclei. Coexpressed with Prox1, but not Pdpn.
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Specimen
1F:
embryonic day 12.5
(more )
Note: Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
Present |
Regionally restricted |
1F
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Expressed in jugular lymph sacs endothelial cells. Expressed in nuclei.
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| TS20: cardinal vein |
Present |
Regionally restricted |
1F
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Expressed in endothelial cells. Expressed in nuclei.
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| TS20: venous system |
Present |
Single cells |
1F
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Expressed in a few jugular vein endothelial cells, which are Prox1-negative. Expressed in nuclei.
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Specimen
1G:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
1G
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Expressed in the trunk lymphatics. Expressed in nuclei. Coexpressed with Prox1 and Flt4.
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Specimen
1H:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
1H
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Expressed in the lung lymphatics. Expressed in nuclei. Coexpressed with Prox1 and Flt4.
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Specimen
1I:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
1I
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Expressed in the dermal lymphatics. Expressed in nuclei. Coexpressed with Prox1 and Flt4.
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Specimen
1J:
postnatal day 21
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
1J:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: lymphatic vessel endothelium |
Present |
Regionally restricted |
1J
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Expressed in the pulmonary lymphatics. Expressed in nuclei. Coexpressed with Prox1 and Flt4.
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Specimen
2A:
embryonic day 11.5
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cardinal vein |
Present |
Regionally restricted |
2A
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Expressed in endothelial cells. Coexpressed with Prox1 and Flt4.
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| TS19: lymphatic system |
Present |
Regionally restricted |
2A
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Expressed in lymphatic endothelial cells sprouting from the cardinal vein. Coexpressed with Prox1 and Flt4.
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Specimen
2B:
embryonic day 11.5
(more )
Note: Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: cardinal vein |
Present |
Regionally restricted |
2B
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Expressed in endothelial cells. Coexpressed with Prox1.
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| TS19: lymphatic system |
Present |
Regionally restricted |
2B
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Expressed in lymphatic endothelial cells sprouting from the cardinal vein. Coexpressed with Prox1.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Nfatc1tm1Glm/Nfatc1tm1Glm
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Nfatc1tm1Glm/Nfatc1tm1Glm
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
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Specimen
S1A/C:
embryonic day 12.5
(more )
Note: Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
Specimen
S1A/C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Nfatc1; red - Flt4 (assay MGI:5660554); blue - Prox1 (assay MGI:5660552).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
Present |
Uniform |
S1A
,
S1C
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Uniform bilateral expression in developing jugular lymph sacs endothelial cells and sprouting lymphatic endothelial cells. Coexpressed with Prox1 and Flt4.
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Specimen
S1D:
embryonic day 12.5
(more )
Note: Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
Specimen
S1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Paraffin
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Note:
|
Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
Present |
Regionally restricted |
S1D
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Uniform bilateral expression in developing jugular lymph sacs endothelial cells and sprouting lymphatic endothelial cells. Coexpressed with Prox1 and Pdpn.
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Specimen
S1E:
embryonic day 12.5
(more )
Note: Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
Specimen
S1E:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
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|
Note:
|
Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lymphatic system |
Present |
Uniform |
S1E
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Uniform bilateral expression in developing jugular lymph sacs endothelial cells and sprouting lymphatic endothelial cells. Coexpressed with Prox1.
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Specimen
S1F:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
Specimen
S1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
|
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Paraffin
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Note:
|
Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
S1F
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Expressed in the dermal lymphatics. Coexpressed with Prox1 and Pdpn.
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Specimen
S1G:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
Specimen
S1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
|
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Sex:
|
Not Specified
|
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
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Paraffin
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|
Note:
|
Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
S1G
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Expressed in the dermal lymphatics. Coexpressed with Prox1.
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Specimen
S1H:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
Specimen
S1H:
(close )
|
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: green - Nfatc1; red - Pdpn (assay MGI:5660608); blue - Prox1 (assay MGI:5660552).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
S1H
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Expressed in the pulmonary lymphatics. Coexpressed with Prox1 and Pdpn.
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Specimen
S1I:
embryonic day 14.5
(more )
Note: Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
Specimen
S1I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Triple labeled: green - Nfatc1; red - GSL-B4 isolectin; blue - Prox1 (assay MGI:5660552).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lymphatic system |
Present |
Regionally restricted |
S1I
|
Expressed in the pulmonary lymphatics. Coexpressed with Prox1.
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Analysis Tools