Probe:	prothymosin alpha cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: embryo	Strong	Regionally restricted	1A	Expression is in most tissues.
TS17: chorion	Strong		1A
TS17: yolk sac endoderm	Strong		1A
TS17: yolk sac	Strong	Regionally restricted	1A	Expression is in hematopoietic islands.
TS17: diencephalon	Moderate
TS17: hindbrain	Moderate
TS17: midbrain	Moderate
TS17: telencephalon	Moderate
TS17: extraembryonic component	Weak	Regionally restricted	1A	Expression is in Reichert's membrane.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	1B	Expression is in most tissues.
TS20: liver	Strong		1B	Expression in prehepatocytes is heterogeneous and stronger than in cells of the hematopoetic islands.
TS20: intestine epithelium	Strong		1B
TS20: thymus primordium	Strong		1B
TS20: diencephalon	Moderate		1B
TS20: medulla oblongata	Moderate		1B
TS20: metencephalon	Moderate		1B
TS20: midbrain	Moderate		1B
TS20: telencephalon	Moderate		1B
TS20: lung	Present	Regionally restricted	1B	Expression is in early lung mesoderm.
TS20: trachea	Present		1B
TS20: heart	Absent		1B

Structure	Level	Pattern	Image	Note
TS22: cartilage	Strong		1C
TS22: gut	Strong		1C
TS22: intestine epithelium	Strong		1C
TS22: lung	Strong		1C
TS22: metanephros	Strong		1C
TS22: molar enamel organ	Strong		1C
TS22: salivary gland	Strong		1C
TS22: thymus primordium	Strong		1C
TS22: diencephalon	Moderate		1C
TS22: medulla oblongata	Moderate		1C
TS22: metencephalon	Moderate		1C
TS22: midbrain	Moderate		1C
TS22: telencephalon	Moderate		1C
TS22: liver	Present		1C	Expression in prehepatocytes is heterogeneous and stronger than in cells of the hematopoetic islands.
TS22: vibrissa	Present		1C

Structure	Level	Pattern	Image	Note
TS24: brown fat	Strong		1D
TS24: cartilage	Strong		1D
TS24: cerebral cortex subventricular zone	Strong		1D
TS24: cerebral cortex ventricular layer	Strong		1D
TS24: dorsal root ganglion	Strong		1D
TS24: gut	Strong		1D
TS24: intestine epithelium	Strong		1D
TS24: lung	Strong		1D
TS24: molar enamel organ	Strong		1D
TS24: neocortex	Strong		1D
TS24: salivary gland epithelium	Strong		1D
TS24: trigeminal ganglion	Strong		1D
TS24: liver	Present		1D	Expression in prehepatocytes is heterogeneous and stronger than in cells of the hematopoetic islands.
TS24: metanephros	Strong		1D	The stronger levels were seen in cortical regions.
TS24: epithalamus	Moderate		1D
TS24: thalamus	Moderate		1D
TS24: vibrissa bulb	Present		1D
TS24: salivary gland mesenchyme	Weak		1D

Structure	Level	Pattern	Image	Note
TS26: hair bulb	Strong		2A
TS26: superior colliculus	Strong		2A
TS26: trigeminal ganglion	Strong		2A
TS26: thalamus	Strong	Regionally restricted	2A	Expression is in the dorsal and superior thalamus.
TS26: liver	Present		2A	Expression in prehepatocytes is heterogeneous and stronger than in cells of the hematopoetic islands.
TS26: thymus	Moderate		2A	Higher levels were seen in cells in the subcapsular core.
TS26: brown fat	Present		2A
TS26: olfactory epithelium	Present		2A
TS26: tooth	Present		2A
TS26: vertebra	Present	Regionally restricted	2A	Expression is in specific areas.
TS26: lung	Absent		2A

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Strong		2B
TS26: cerebral cortex ventricular zone	Strong		2B
TS26: cortical plate	Strong		2B
TS26: hair bulb	Strong		2B
TS26: neocortex	Present		2B

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Strong	Regionally restricted	2D	Expression is in the external granular layer, weak in the internal layer, absent in the molecular layer.
TS26: hair bulb	Strong		2D

Structure	Level	Pattern	Image	Note
TS26: intestine epithelium	Present	Regionally restricted	3A	Expression is stronger in the intestinal crypts, lower in non-proliferating cells and differentiating epithelial cells lining the villi.

Structure	Level	Pattern	Image	Note
TS26: nephrogenic zone	Strong		3B
TS26: metanephros	Present		3B	Expression is strongest in the nephrogenic zone and is absent in the central part.
TS26: mesenchyme of metanephros	Present		3B
TS26: renal cortex	Present	Regionally restricted	3B	Expression is in the newly formed epithelia in the upper cortex.
TS26: maturing glomerular tuft	Weak		3B
TS26: renal tubule	Weak	Regionally restricted	3B	Expression is in well-differentiated tubuli.

Structure	Level	Pattern	Image	Note
TS26: salivary gland epithelium	Strong		3C
TS26: salivary gland mesenchyme	Weak		3C

Structure	Level	Pattern	Image	Note
TS26: thymus	Present	Regionally restricted	3D	Expression is in the subcapsular cortex and weaker in the cortical regions.