Gene Expression Data
Assay Details
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Assay
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Reference:
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J:35538
Mallamaci A, et al., OTX2 homeoprotein in the developing central nervous system and migratory cells of the olfactory area. Mech Dev. 1996 Aug;58(1-2):165-78
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5649317
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Antibody:
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anti-OTX2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Tissue was fixed in Histochoice.
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Results
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Specimen
4d:
embryonic day 17.0-17.5
(more  )
Note: Sagittal section. Double labeled: gray/brown - Otx2; blue - Calb1 (assay MGI:5649355).
Specimen
4d:
(close  )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 17.0-17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Sagittal section. Double labeled: gray/brown - Otx2; blue - Calb1 (assay MGI:5649355).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: superior colliculus |
Present |
Regionally restricted |
4d
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Expression is present in cells of superficial gray.
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Specimen
5g:
embryonic day 17.5
(more )
Note: Double labeled: brown - Otx2; blue - Calb1 (assay MGI:5649355).
Specimen
5g:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Otx2; blue - Calb1 (assay MGI:5649355).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebellum |
Present |
Regionally restricted |
5g
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Expression is present in the granule precursors of the external granular layer.
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Specimen
7h:
embryonic day 12.0
(more )
Note: Transverse section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
Specimen
7h:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Transverse section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: olfactory epithelium |
Present |
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7h
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Expression is mainly restricted to nuclei lying in luminal position.
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| TS20: nasal mesenchyme |
Present |
Regionally restricted |
7h
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Expression is arrayed in cylindrical structures that appear to ensheath the axon bundles of the olfactory and vomeronasal nerves, along the trajectories they follow from the medial main olfactory bulb and vomeronasal organ to the olfactory bulb.
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Specimen
7i:
embryonic day 12.0
(more )
Note: Transverse section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
Specimen
7i:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Transverse section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: olfactory epithelium |
Present |
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7i
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Expression is mainly restricted to nuclei lying in luminal position.
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| TS20: nasal mesenchyme |
Present |
Regionally restricted |
7i
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Expression is arrayed in cylindrical structures that appear to ensheath the axon bundles of the olfactory and vomeronasal nerves, along the trajectories they follow from the medial main olfactory bulb and vomeronasal organ to the olfactory bulb.
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Specimen
7j/k:
embryonic day 12.0
(more )
Note: Sagittal section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
Specimen
7j/k:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Sagittal section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nasal mesenchyme |
Present |
Regionally restricted |
7k
,
7j
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Expression is arrayed in cylindrical structures that appear to ensheath the axon bundles of the olfactory and vomeronasal nerves, along the trajectories they follow from the medial main olfactory bulb and vomeronasal organ to the olfactory bulb.
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| TS20: nose |
Present |
Regionally restricted |
7j
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7k
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Expression is present in scattered cells localized just ventral to the anlage of the olfactory bulb. These cells are tightly associated with the most distal parts of the olfactory axons growing toward the olfactory bulb.
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| TS20: olfactory epithelium |
Present |
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7k
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7j
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Specimen
7l/m:
embryonic day 12.0
(more )
Note: Sagittal section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
Specimen
7l/m:
(close )
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Genetic Background:
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involves: C57BL/6 * DBA/2
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Sagittal section. Double labeled: brown - Otx2; dark violet/black - Mapt (Tau; assay MGI:5649368).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: nasal mesenchyme |
Present |
Regionally restricted |
7l
,
7m
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Expression is arrayed in cylindrical structures that appear to ensheath the axon bundles of the olfactory and vomeronasal nerves, along the trajectories they follow from the medial main olfactory bulb and vomeronasal organ to the olfactory bulb.
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| TS20: olfactory epithelium |
Present |
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7l
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7m
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