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Gene Expression Data
Assay Details
Assay
Reference: J:188473 Kan-o M, et al., Expression and subcellular localization of mammalian formin Fhod3 in the embryonic and adult heart. PLoS One. 2012;7(4):e34765
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5648945
Gene symbol: Fhod3
Gene name: formin homology 2 domain containing 3
Antibody: Anti-Fhod3 (C-20)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Fixation: 3.7% paraformaldehyde.
Results
Specimen B bottom: postnatal adult (more )
Note: Double labeled: red - Fhod3; green - Actn2 (assay MGI:5648946). The antibody was treated with an acetone powder of mouse embryonic fibroblasts derived from Fhod3 knockout mice.
Structure Level Pattern Image Note
TS28: heart Present 3B Fhod3 bottom , 3B merge bottom Expression was detected in the sarcomere as a doublet. Treating the antibody with an acetone powder of mouse embryonic fibroblasts derived from knockout mice attenuated signal in the Z-line.
Specimen S1: postnatal adult (more )
Note: Double labeled: red - Fhod3; green - Actn2 (assay MGI:5648946).
Structure Level Pattern Image Note
TS28: heart Present S1_merge , S1_untreated Fhod3 (C-20) Expression was detected in the Z-line without pre-treatment of the antibody with acetone powder of mouse embryonic fibroblasts derived from Fhod3 knockout mice.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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