Gene Expression Data
Assay Details
Assay
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Reference:
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J:38660
Worrad DM, et al., Regulation of gene expression in the preimplantation mouse embryo: temporal and spatial patterns of expression of the transcription factor Sp1. Mol Reprod Dev. 1997 Mar;46(3):268-77
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5648732
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Gene symbol:
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Sp1
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Gene name:
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trans-acting transcription factor 1
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Antibody:
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anti-Sp1
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Quantification of immunocytochemical analyses was shown in Fig. 5.
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Results
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Specimen
4A:
embryonic day 0.5
(more )
Note: One-cell embryos in G1.
Specimen
4A:
(close )
Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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One-cell embryos in G1.
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Specimen
4B:
embryonic day 0.5
(more )
Note: One-cell embryos in G2.
Specimen
4B:
(close )
Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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One-cell embryos in G2.
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Specimen
4C:
embryonic day 1.0
(more )
Note: Two-cell embryos in G2.
Specimen
4C:
(close )
Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Two-cell embryos in G2.
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Specimen
4D:
embryonic day 2.5
(more )
Note: Eight-cell embryos.
Specimen
4D:
(close )
Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Eight-cell embryos.
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Specimen
4E:
embryonic day 3.5
(more )
Note: Blastocysts.
Specimen
4E:
(close )
Genetic Background:
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involves: C57BL/6J * CF-1 * DBA/2J
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Note:
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Blastocysts.
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Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst-stage conceptus |
Present |
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4E
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The total amount increased between the one-cell and blastocysts stages. The nuclear concentration in the trophectoderm cells was greater than in the inner cell mass cells.
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