Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81188
Velasco-Miguel S, et al., Constitutive and regulated expression of the mouse Dinb (Polkappa) gene encoding DNA polymerase kappa. DNA Repair (Amst). 2003 Jan 2;2(1):91-106
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5648413
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Gene symbol:
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Polk
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Gene name:
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polymerase (DNA directed), kappa
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Antibody:
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DinB antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
2D:
postnatal adult
(more  )
Specimen
2D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary spermatocyte |
Present |
Regionally restricted |
2D
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Expression is in pachytene cells in meiosis I.
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| TS28: spermatid |
Present |
Regionally restricted |
2D
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Expression is in post-meiotic round spermatids.
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Specimen
5E:
postnatal adult
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Polktm1.1Rsky/Polktm1.1Rsky
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
6A:
postnatal adult
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Polktm1.1Rsky/Polktm1.1Rsky
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
6C:
postnatal adult
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Polktm1.1Rsky/Polktm1.1Rsky
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Specimen
6E:
postnatal adult
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: stomach epithelium |
Present |
Regionally restricted |
6E
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Expression is in mucus and chief cells in the epithelium of the stomach, but not in the acid-secreting parietal cells.
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Specimen
not shown male:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Polktm1.1Rsky/Polktm1.1Rsky
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown female:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Polktm1.1Rsky/Polktm1.1Rsky
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Polktm1.1Rsky/Polktm1.1Rsky
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools