Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cone	Present	Regionally restricted	1A	Expression in trophoblast cells covering surface of ectoplacental cone.
TS10: parietal endoderm	Present	Regionally restricted	1A	Expression in subpopulation of parietal endoderm cells.
TS10: embryo mesoderm	Present	Regionally restricted	1A	Expression in anterior head region mesoderm as two small streaks underlying neural folds. In posterior mesoderm, expression lateral to primitive streak.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	1B far left	Expression in trophoblast cells covering surface of ectoplacental cone.
TS11: parietal endoderm	Present	Regionally restricted	1B far left	Expression in smaller parietal endoderm cells underlying parietal yolk sac.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	1B middle left	Expression in trophoblast cells covering surface of ectoplacental cone.
TS11: mesenchyme derived from head mesoderm	Present	Regionally restricted	1B middle left	Expression in laterally expanding mesoderm of head region. Expression in anterior mesoderm apposed to prospective neural fold ectoderm became stronger and more widespread as cell population expanded laterally.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	1B middle right	Expression in trophoblast cells covering surface of ectoplacental cone.
TS11: mesenchyme derived from head mesoderm	Present	Regionally restricted	1B middle right	Expression in laterally expanding mesoderm of head region. Expression in anterior mesoderm apposed to prospective neural fold ectoderm became stronger and more widespread as cell population expanded laterally.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	1B far right	Expression in trophoblast cells covering surface of ectoplacental cone.
TS11: mesenchyme derived from head mesoderm	Present	Regionally restricted	1B far right	Expression in laterally expanding mesoderm of head region. Expression in anterior mesoderm apposed to prospective neural fold ectoderm became stronger and more widespread as cell population expanded laterally.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present	Regionally restricted	1C	Expression in trophoblast cells covering surface of ectoplacental cone.
TS11: mesenchyme derived from head mesoderm	Present	Regionally restricted	1C	Expression in laterally expanding mesoderm of head region. Expression in anterior mesoderm apposed to prospective neural fold ectoderm became stronger and more widespread as cell population expanded laterally.

Structure	Level	Pattern	Image	Note
TS11: yolk sac mesoderm	Weak	Spotted		Weak speckled expression in mesoderm of visceral yolk sac

Structure	Level	Pattern	Image	Note
TS13: parietal endoderm	Present	Punctate	1D	Punctate expression in a majority of small, round parietal endoderm cells, covering the embryonic aspect of Reicherts membrane.
TS13: trophectoderm	Present	Regionally restricted	1D	Expression associated with trophoblast cells overlaying the mesometrial pole.
TS13: secondary trophoblast giant cell	Strong	Regionally restricted	1D	Intense expression associated with individual secondary giant trophoblast cells attached to the maternal surface of Reicherts membrane.

Structure	Level	Pattern	Image	Note
TS13: secondary trophoblast giant cell	Strong	Regionally restricted	1E	Intense expression associated with individual secondary giant trophoblast cells attached to the maternal surface of Reicherts membrane.
TS13: allantois endothelium	Present	Regionally restricted	1E	Expression in endothelial cells in allantois, fused to chorionic plate. Expression in cluster of cells in allantoic stalk, connected with intraembryonic blood vessels.
TS13: mesenchyme derived from head mesoderm	Present	Regionally restricted	1E	Expression throughout angioblastic mesenchyme, including sprouting blood vessels establishing the perineural plexus.
TS13: endocardial tube	Present		1E
TS13: heart mesenchyme	Present		1E
TS13: dorsal aorta	Present	Widespread	1E	Expression in paired dorsal aorta over its entire length.

Structure	Level	Pattern	Image	Note
TS13: secondary trophoblast giant cell	Strong	Regionally restricted	1F	Intense expression associated with individual secondary giant trophoblast cells attached to the maternal surface of Reicherts membrane.
TS13: mesenchyme derived from head mesoderm	Present	Regionally restricted	1F	Expression throughout angioblastic mesenchyme, including sprouting blood vessels establishing the perineural plexus.
TS13: endocardial tube	Present		1F
TS13: heart mesenchyme	Present		1F
TS13: allantois endothelium	Present	Regionally restricted	1F	Expression in endothelial cells in allantois, fused to chorionic plate, in cluster of cells in allantoic stalk, connected with intraembryonic blood vessels.
TS13: dorsal aorta	Present	Widespread	1F	Expression in paired dorsal aorta over its entire length.

Structure	Level	Pattern	Image	Note
TS13: secondary trophoblast giant cell	Strong	Regionally restricted	1G	Intense expression associated with individual secondary giant trophoblast cells attached to the maternal surface of Reicherts membrane.
TS13: yolk sac blood vessel	Strong	Regionally restricted	1G	Stronger expression in vascular endothelium of yolk sac as individual blood islands merge to form a capillary plexus.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Present	Widespread		Expression in coherent layer of trophoblast cells surrounding entire embryo.

Structure	Level	Pattern	Image	Note
TS15: yolk sac blood vessel	Present	Uniform	1H	Homogenous expression in endothelium of yolk sac vasculature.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	1I	Expression in mesencephalic flexure.
TS15: vascular element	Present	Regionally restricted	1I	Homogenous expression in endothelium of all intraembryonic vessels.

Structure	Level	Pattern	Image	Note
TS15: endocardial cushion	Strong	Regionally restricted	3A	Intense expression of the tissue forming the atrioventricular valve.
TS15: endocardial lining	Present		3A
TS15: cardiac muscle tissue	Absent		3A
TS15: dorsal aorta	Present	Regionally restricted	3A	Expression in endothelium of dorsal aorta.
TS15: trunk mesenchyme	Present	Regionally restricted	3A	Expression in the mesenchyme / submucosal tissue surrounding esophagus and trachea.
TS15: tracheal diverticulum epithelium	Absent		3A	No expression in airway epithelium of the tracheal bifurcation.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	3B	Expression in ventral midline of mesencephalic flexure epithelium.
TS15: head mesenchyme	Present	Regionally restricted	3B	Expression in leptomeninges.

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme	Present	Regionally restricted	3C	Expression in submucosal tissue surrounding trachea and bronchi.
TS16: bronchus epithelium	Absent		3C

Structure	Level	Pattern	Image	Note
TS16: lung epithelium	Present	Regionally restricted	3D	Expression in lung bud epithelium in more caudal, less mature regions of the lung.