Probe:	Evi-1 probe5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: nephric duct	Present	Widespread	2A/B	Expression detected throughout the mesonephric duct.
TS15: mesonephric tubule	Present		2A/B
TS15: somite	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS20: mesonephric tubule	Strong		2C/D
TS20: nephric duct, mesonephric portion	Present		2C/D	Expression in the Wolffian duct (caudal portion of the mesonephric duct).
TS20: mesonephros mesenchyme	Present	Regionally restricted	2C/D	Distinct ring of expression in mesenchyme surrounding the more caudal end of the Wolffian duct.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Strong		2E/F
TS22: testis	Absent		2E/F

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Absent

Structure	Level	Pattern	Image	Note
TS20: paramesonephric duct	Present		3A/B	Expression in the Mullerian duct.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Widespread	4A/B	Expression clearly seen throughout non-ectodermal cells of the limb.
TS15: forelimb bud presumptive apical ectodermal ridge	Absent		4A/B
TS15: somite	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Uniform	4C/D
TS20: trunk mesenchyme	Present	Regionally restricted	4C/D	Expression extends into lateral mesoderm in areas destined to form musculoskeleton on limb girdle.

Structure	Level	Pattern	Image	Note
TS22: perichondrium	Present	Regionally restricted	4E/F	Expression limited to perichondrium of limb cartilage.

Structure	Level	Pattern	Image	Note
TS22: foot mesenchyme	Present	Regionally restricted	4G/H	Expression limited to perichondrium of limb cartilage.
TS22: hindlimb interdigital region mesenchyme	Present		4G/H

Structure	Level	Pattern	Image	Note
TS20: outflow tract	Strong	Regionally restricted	5A/B	High expression in both truncal and conal ridges, and in the cardiac valves.
TS20: blood vessel	Absent		5A/B
TS20: cardiac muscle tissue	Absent		5A/B
TS20: endocardial lining	Absent		5A/B
TS20: bronchus epithelium	Present		5A/B
TS20: trachea epithelium	Present		5A/B
TS20: esophagus	Absent		5A/B
TS20: lung mesenchyme	Absent		5A/B
TS20: oral epithelium	Absent		5A/B
TS20: visceral pleura	Absent		5A/B
TS20: hyoid cartilage condensation	Present		5A/B
TS20: mandible primordium	Present		5A/B

Structure	Level	Pattern	Image	Note
TS22: heart	Absent		5C/D
TS22: bronchiole	Present	Regionally restricted	5C/D	Expression outlines developing trachea, bronchi and bronchioles.
TS22: bronchus	Present	Regionally restricted	5C/D	Expression outlines developing trachea, bronchi and bronchioles.
TS22: trachea	Present	Regionally restricted	5C/D	Expression outlines developing trachea, bronchi and bronchioles.

Structure	Level	Pattern	Image	Note
TS20: vomeronasal organ epithelium	Strong	Regionally restricted	6A/B	High level of expression in epithelial layer of medial invagination of nasoepithelium that will form the vomeromedial organ of Jacobson.
TS20: nasal mesenchyme	Present	Regionally restricted	6A/B	Significant level of expression in a very localized region of mesenchyme lateral to each nasal cavity, at a fold in lateral wall of nasal chamber, which may coincide with formation of conchae.