Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149243
Wilhelm D, et al., Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev. 2009 May-Jun;126(5-6):324-36
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5646220
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Gene symbol:
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Foxl2
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Gene name:
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forkhead box L2
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Antibody:
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Anti-Foxl2 (C terminus)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Assay notes:
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Two antibodies were described in the reference for this antibody - MGI:5618299 and MGI:5640581. Either could have been used in this assay.
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Results
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Specimen
5B:
embryonic day 13.5; X/ SryPOS
(more  )
Note: XY ovotestes. Double labeled: red - Foxl2; green - Sox9 (assay MGI:5646130).
Specimen
5B:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: red - Foxl2; green - Sox9 (assay MGI:5646130).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: interstitium of the testis |
Present |
Regionally restricted |
5B
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Expression in the morphological testis tissue in the center was in the interstitium between Sox9-positive testis cords. No double-positive cells were observed.
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Analysis Tools
