Gene Expression Data
Assay Details
Assay
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Reference:
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J:149243
Wilhelm D, et al., Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev. 2009 May-Jun;126(5-6):324-36
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5646133
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Gene symbol:
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Nr5a1
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Gene name:
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nuclear receptor subfamily 5, group A, member 1
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Antibody:
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Anti-SF1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
6A:
embryonic day 11.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Specimen
6A:
(close )
Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: gonad |
Strong |
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6A
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Expression was detected in somatic cells along the whole length of the gonad.
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Specimen
6B:
embryonic day 12.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Specimen
6B:
(close )
Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: gonad |
Strong |
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6B
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Expression was detected in somatic cells along the whole length of the gonad, including regions in which the ovarian pathway was initiated.
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Specimen
6C:
embryonic day 13.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Specimen
6C:
(close )
Genetic Background:
|
involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: gonad |
Present |
Regionally restricted |
6C
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From E13.5 onwards, expression was decreased in Foxl2-positive regions that had committed to the ovarian fate (as indicated by staining in adjacent sections).
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Specimen
6D:
embryonic day 14.5; X/ SryPOS
(more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Specimen
6D:
(close )
Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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X/SryPOS
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: gonad |
Present |
Regionally restricted |
6D
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Expression was decreased in Foxl2-positive regions that had committed to the ovarian fate (as indicated by staining in adjacent sections).
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