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Gene Expression Data
Assay Details
Assay
Reference: J:149243 Wilhelm D, et al., Antagonism of the testis- and ovary-determining pathways during ovotestis development in mice. Mech Dev. 2009 May-Jun;126(5-6):324-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5646133
Gene symbol: Nr5a1
Gene name: nuclear receptor subfamily 5, group A, member 1
Antibody: Anti-SF1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6A: embryonic day 11.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS19: gonad Strong 6A Expression was detected in somatic cells along the whole length of the gonad.

Specimen 6B: embryonic day 12.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS20: gonad Strong 6B Expression was detected in somatic cells along the whole length of the gonad, including regions in which the ovarian pathway was initiated.

Specimen 6C: embryonic day 13.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS21: gonad Present Regionally restricted 6C From E13.5 onwards, expression was decreased in Foxl2-positive regions that had committed to the ovarian fate (as indicated by staining in adjacent sections).

Specimen 6D: embryonic day 14.5; X/SryPOS (more )
Note: XY ovotestes. Double labeled: green - Nr5a1; red - Cdh1 (assay MGI:5646129).
Structure Level Pattern Image Note
TS22: gonad Present Regionally restricted 6D Expression was decreased in Foxl2-positive regions that had committed to the ovarian fate (as indicated by staining in adjacent sections).


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory