Antibody:	anti-TGFb1 (V)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: trophoblast giant cell	Present	Regionally restricted	1A
TS28: decidua	Present	Regionally restricted	1A

Structure	Level	Pattern	Image	Note
TS13: heart	Present		2A

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber cardiac muscle	Strong		2B
TS15: primitive ventricle cardiac muscle	Strong		2B
TS15: embryo mesenchyme	Weak		2B

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber cardiac muscle	Strong		2C
TS15: primitive ventricle cardiac muscle	Strong		2C
TS15: embryo mesenchyme	Weak		2C

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		2D

Structure	Level	Pattern	Image	Note
TS20: somite	Present	Regionally restricted	3A	Expression is in the epithelium.
TS20: sclerotome	Present		3A

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	3B	Expression is in maturing cartilage, chondrocytes, and perichondrium.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present	Regionally restricted	3C	Expression is in the nerve trunks.
TS21: spinal component of peripheral nervous system	Present	Regionally restricted	3C	Expression is in the nerve trunks.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present		3D
TS21: brain	Weak	Regionally restricted	3D	Expression is in the areas surrounding the choroid plexus.

Structure	Level	Pattern	Image	Note
TS22: bronchus epithelium	Present		4A
TS22: lung epithelium	Present		4A
TS22: lung vascular element	Present		4A

Structure	Level	Pattern	Image	Note
TS22: liver	Present		4B	Expression is in megakaryocytes and in hepatocytes.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present		4C
TS22: intestine smooth muscle	Present		4C

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present		4D

Structure	Level	Pattern	Image	Note
TS24: cartilage	Strong		4I
TS24: esophagus epithelium	Strong		4I
TS24: trachea epithelium	Strong		4I

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Strong		4J
TS24: intestine muscularis	Strong		4J

Structure	Level	Pattern	Image	Note
TS13: heart	Present

Structure	Level	Pattern	Image	Note
TS15: heart	Present

Structure	Level	Pattern	Image	Note
TS17: arterial system	Strong
TS17: heart	Strong
TS17: brain	Present
TS17: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS19: heart	Very strong
TS19: arterial system	Strong
TS19: brain	Present
TS19: dorsal root ganglion	Present
TS19: future pituitary	Present
TS19: future spinal cord	Present
TS19: intestine epithelium	Present
TS19: lung epithelium	Present
TS19: trachea epithelium	Present
TS19: intestine	Present	Regionally restricted		Expression is in the muscle.
TS19: liver	Weak	Regionally restricted		Weak in megakaryocytes, absent in hepatocytes.

Structure	Level	Pattern	Image	Note
TS20: heart	Very strong
TS20: arterial system	Strong
TS20: dorsal root ganglion	Strong
TS20: pituitary gland	Strong
TS20: skeletal muscle	Strong
TS20: spinal cord	Strong
TS20: brain	Present
TS20: bronchus epithelium	Present
TS20: choroid plexus	Present
TS20: intestine epithelium	Present
TS20: lung epithelium	Present
TS20: lung mesenchyme	Present
TS20: trachea epithelium	Present
TS20: skeleton	Present	Regionally restricted		Expression is in perichondrium and in chondrocytes.
TS20: intestine	Present	Regionally restricted		Expression is in the muscle.
TS20: liver	Present	Regionally restricted		Expression is in hepatocytes, stronger in megakaryocytes.
TS20: metanephros	Weak	Regionally restricted		Expression is in metanephric duct derivatives, absent in glomeruli.

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Very strong
TS21: heart	Very strong
TS21: spinal cord	Very strong
TS21: adrenal gland	Strong
TS21: arterial system	Strong
TS21: choroid plexus	Strong
TS21: pituitary gland	Strong
TS21: skeletal muscle	Strong
TS21: thymus primordium	Strong
TS21: trachea epithelium	Strong
TS21: liver	Strong			Expression is in hepatocytes, stronger in megakaryocytes.
TS21: metanephros	Strong	Regionally restricted		Expression is in metanephric duct derivatives, absent in glomeruli.
TS21: brain	Present
TS21: intestine epithelium	Present
TS21: intestine muscularis	Present
TS21: lung epithelium	Present
TS21: lung mesenchyme	Present
TS21: skeleton	Present	Regionally restricted		Expression is in perichondrium, in chondrocytes, and in hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Very strong
TS22: choroid plexus	Very strong
TS22: dorsal root ganglion	Very strong
TS22: heart	Very strong
TS22: intestine epithelium	Very strong
TS22: spinal cord	Very strong
TS22: thymus primordium	Very strong
TS22: trachea epithelium	Very strong
TS22: smooth muscle tissue	Very strong	Regionally restricted		Expression is in airway smooth muscle cells.
TS22: metanephros	Very strong	Regionally restricted		Expression is in metanephric duct derivatives.
TS22: liver	Strong			Expression is in hepatocytes, stronger in megakaryocytes.
TS22: arterial system	Strong
TS22: intestine muscularis	Strong
TS22: maturing glomerular tuft	Strong
TS22: pituitary gland	Strong
TS22: skeletal muscle	Strong
TS22: brain	Present
TS22: bronchus epithelium	Present
TS22: lung epithelium	Present
TS22: lung mesenchyme	Present
TS22: skeleton	Present	Regionally restricted		Expression is in perichondrium, in chondrocytes, and in hypertrophic cartilage.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Very strong
TS23: dorsal root ganglion	Very strong
TS23: heart	Very strong
TS23: intestine epithelium	Very strong
TS23: skeletal muscle	Very strong
TS23: spinal cord	Very strong
TS23: thymus primordium	Very strong
TS23: trachea epithelium	Very strong
TS23: smooth muscle tissue	Very strong	Regionally restricted		Expression is in airway smooth muscle cells.
TS23: metanephros	Very strong	Regionally restricted		Expression is in metanephric duct derivatives.
TS23: arterial system	Strong
TS23: choroid plexus	Strong
TS23: intestine muscularis	Strong
TS23: maturing glomerular tuft	Strong
TS23: pituitary gland	Strong
TS23: liver	Strong	Regionally restricted		Expression is in hepatocytes, stronger in megakaryocytes.
TS23: brain	Present
TS23: lung epithelium	Present
TS23: lung mesenchyme	Present
TS23: skeleton	Present	Regionally restricted		Expression is in perichondrium, in chondrocytes, in hypertrophic cartilage, and in osteoblasts.

Structure	Level	Pattern	Image	Note
TS24: adrenal gland	Very strong
TS24: bronchus epithelium	Very strong
TS24: dorsal root ganglion	Very strong
TS24: heart	Very strong
TS24: intestine epithelium	Very strong
TS24: skeletal muscle	Very strong
TS24: spinal cord	Very strong
TS24: thymus	Very strong
TS24: trachea epithelium	Very strong
TS24: smooth muscle tissue	Very strong	Regionally restricted		Expression is in airway smooth muscle cells.
TS24: metanephros	Very strong	Regionally restricted		Expression is in metanephric duct derivatives.
TS24: arterial system	Strong
TS24: choroid plexus	Strong
TS24: intestine muscularis	Strong
TS24: maturing glomerular tuft	Strong
TS24: liver	Strong	Regionally restricted		Expression is in hepatocytes, stronger in megakaryocytes.
TS24: brain	Present
TS24: lung epithelium	Present
TS24: lung mesenchyme	Present
TS24: skeleton	Present	Regionally restricted		Expression is in perichondrium, in chondrocytes, in hypertrophic cartilage, and in osteoblasts.