Probe:	Cygb probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		2A/D

Structure	Level	Pattern	Image	Note
TS21: pituitary gland	Present		2B/E
TS21: nasal cavity	Present	Regionally restricted	2B/E	Expression in submucosa of oral-nasal cavity.
TS21: oral cavity	Present	Regionally restricted	2B/E	Expression in submucosa of oral-nasal cavity.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	2C/F	Expression in subventricular zone of dorsal anterior thalamus.
TS24: cerebral cortex	Present	Regionally restricted	2C/F	Expression in anteo-lateral ergion of cortex.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Moderate		3B
TS20: lingual swelling	Moderate		3B
TS20: spinal cord	Moderate	Regionally restricted	3B	Moderate expression in ventral portion of neural tube.
TS20: heart	Absent		3B
TS20: skeletal muscle	Absent		3B
TS20: somite	Absent		3B

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Regionally restricted		Focal expression in dermal epithelium.

Structure	Level	Pattern	Image	Note
TS28: paraventricular thalamic nucleus	Moderate	Punctate	5A/B

Structure	Level	Pattern	Image	Note
TS28: suprachiasmatic nucleus	Moderate	Punctate	5C/D
TS28: supraoptic nucleus	Present		5C/D

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Moderate	Punctate	5E/F

Structure	Level	Pattern	Image	Note
TS28: solitary tract nucleus	Moderate	Punctate	5G/H

Structure	Level	Pattern	Image	Note
TS28: solitary tract nucleus	Present	Regionally restricted	5I/J	Expression localized to neurons.

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Present	Regionally restricted	6A	Punctate expression especially in lateral and posterior areas.
TS28: pallidum	Present	Regionally restricted	6A	Puncate expression in the ventral pallidum.
TS28: nucleus of diagonal band	Present	Regionally restricted	6A	Expression in the nucleus of vertical limb of diagonal band.

Structure	Level	Pattern	Image	Note
TS28: medial preoptic nucleus	Present		6C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		6E
TS28: medial habenular nucleus	Strong		6E
TS28: reticular thalamic nucleus	Strong		6E
TS28: hippocampus pyramidal cell layer	Weak	Punctate	6E	Weaker, punctate expression throughout the pyramidal cell layer of CA1, CA2 and CA3.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		6G
TS28: hippocampus pyramidal cell layer	Weak	Punctate	6G	Weaker, punctate expression throughout the pyramidal cell layer of CA1, CA2 and CA3.
TS28: paraventricular thalamic nucleus	Present		6G

Structure	Level	Pattern	Image	Note
TS28: cochlear nucleus	Present		6I
TS28: lateral reticular nucleus	Present		6I
TS28: locus coeruleus	Present		6I
TS28: medial vestibular nucleus	Present		6I
TS28: solitary tract nucleus	Present		6I

Structure	Level	Pattern	Image	Note
TS28: cochlear nucleus	Present		6K
TS28: lateral reticular nucleus	Present		6K
TS28: locus coeruleus	Present		6K
TS28: medial vestibular nucleus	Present		6K
TS28: solitary tract nucleus	Present		6K

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Strong	Diffuse
TS28: insular cortex	Strong	Diffuse
TS28: primary motor cortex	Strong	Diffuse
TS28: anterior dorsal thalamic nucleus	Strong
TS28: interpeduncular nucleus	Strong
TS28: lateral mammillary nucleus	Strong
TS28: piriform cortex	Strong
TS28: pontine nucleus	Strong
TS28: supramammillary nucleus	Strong
TS28: vestibular nucleus	Strong
TS28: caudate-putamen	Present	Punctate
TS28: hippocampus CA3	Present	Punctate
TS28: basomedial amygdaloid nucleus	Present
TS28: dorsal cochlear nucleus	Present
TS28: dorsomedial hypothalamic nucleus	Present
TS28: frontal cortex	Present
TS28: hypothalamus lateral zone	Present
TS28: indusium griseum	Present
TS28: lateral habenular nucleus	Present
TS28: main olfactory bulb granule cell layer	Present
TS28: main olfactory bulb mitral cell layer	Present
TS28: medial mammillary nucleus	Present
TS28: medial preoptic area	Present
TS28: medial septal nucleus	Present
TS28: median raphe nucleus	Present
TS28: posterior thalamic nucleus	Present
TS28: red nucleus	Present
TS28: trigeminal V motor nucleus	Present
TS28: trigeminal V sensory nucleus	Present
TS28: globus pallidus	Present	Regionally restricted		Expression in lateral globus pallidus.
TS28: accumbens nucleus	Present	Regionally restricted		Expression in accumbens shell (lateral region).
TS28: endopiriform nucleus	Present	Regionally restricted		Expression in dorsal endopiriform nucleus.
TS28: lateral septal nucleus	Present	Regionally restricted		Expression in lateral septal nucleus (intermediate).
TS28: lateral hypothalamic area	Present	Regionally restricted		Expression in lateral anterior hypothalamic nucleus.
TS28: telencephalon	Present	Regionally restricted		Authors also report expression in anterior optic nucleus (ventral) and dorsal peducular cortex.
TS28: diencephalon	Present	Regionally restricted		Authors also report expression in anterior pretectal nucleus (dorsal), interstitial nucleus of Cajal, magnocellular preoptic nucleus and premammillary nucleus.
TS28: hindbrain	Present	Regionally restricted		Authors also report expression in nucleus of roller, prepositus nucleus, reticular nucleus and rostroventricular reticular nucleus.

Structure	Level	Pattern	Image	Note
TS28: brain gray matter	Present
TS28: brain blood vessel	Absent

Structure	Level	Pattern	Image	Note
TS28: amygdala	Weak	Punctate	8A

Structure	Level	Pattern	Image	Note
TS28: peduncular pontine nucleus	Present		8C