Antibody:	ODC-29
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two antibodies were described - MGI:5645493 and MGI:5645491 , but the one used in the assay was not specified.

Structure	Level	Pattern	Image	Note
TS12: conceptus	Present

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1J	Expression was in neurons.

Structure	Level	Pattern	Image	Note
TS21: lens fiber	Present	Regionally restricted	2A	Expression was in primary lens fiber cells.
TS21: cornea	Weak		2A
TS21: lens epithelium	Weak		2A
TS21: pigmented retinal epithelium	Weak		2A
TS21: retina	Weak		2A

Structure	Level	Pattern	Image	Note
TS26: lens fiber	Present	Regionally restricted	2D	Expression was detected in the secondary lens fiber cells.
TS26: neural retina	Present		2D

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted		Expression was detected in the secondary lens fibers.
TS28: ciliary body	Present			Expression was detected in the ciliary process.
TS28: neural retina	Present
TS28: pigmented retinal epithelium	Present

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present		2F
TS28: iris	Present		2F

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer	Present		2G
TS28: pigmented retinal epithelium	Present		2G
TS28: retina ganglion cell layer	Present		2G
TS28: retina inner plexiform layer	Present		2G
TS28: retina outer plexiform layer	Present		2G
TS28: optic choroid	Present	Regionally restricted	2G	Expression was detected in the pigmented vascularized choroid.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present
TS17: spinal nerve	Present

Structure	Level	Pattern	Image	Note
TS18: liver	Weak
TS18: cranial nerve	Present	Regionally restricted		Expression was detected in the infraorbital nerve, a maxillary branch from the trigeminal ganglion.
TS18: embryo mesenchyme	Present
TS18: head mesenchyme	Present
TS18: surface ectoderm	Present

Structure	Level	Pattern	Image	Note
TS23: liver	Present	Single cells

Structure	Level	Pattern	Image	Note
TS26: brown fat	Present
TS26: liver	Present
TS26: gut	Present	Graded		Expression exhibited a proximo-distal gradient; in some distal segments, expression was barely detectable.

Structure	Level	Pattern	Image	Note
TS27: brown fat	Trace
TS27: liver	Trace

Structure	Level	Pattern	Image	Note
TS28: brown fat	Trace
TS28: liver	Trace

Structure	Level	Pattern	Image	Note
TS28: liver	Present
TS28: renal tubule	Present
TS28: glomerular tuft	Absent

Structure	Level	Pattern	Image	Note
TS26: enteric ganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS26: intestinal villus	Present	Regionally restricted	3F	Expression was detected in the most distal part of the villi.

Structure	Level	Pattern	Image	Note
TS22: ureteric tip	Present		3H

Structure	Level	Pattern	Image	Note
TS26: renal tubule	Present		3I
TS26: nephrogenic zone	Absent		3I	No immunostaining was detectable in the tubules of the nephrogenic zone.
TS26: renal corpuscle	Absent		3I

Structure	Level	Pattern	Image	Note
TS22: testis	Present	Regionally restricted	3L	Expression was detected in the seminiferous tubules.
TS22: testis mesenchyme	Trace		3L

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Strong	Regionally restricted	4L	Expression was localized in the epithelium of terminal bronchi.
TS22: lobar bronchus	Ambiguous		4L	Expression was virtually negative.
TS22: lung mesenchyme	Ambiguous		4L	Expression was virtually negative.

Structure	Level	Pattern	Image	Note
TS26: lung	Present			There was a general decrease in expression except in some cells that are likely differentiated pneumocytes.

Structure	Level	Pattern	Image	Note
TS27: alveolar system	Present		4O
TS27: bronchiole	Present		4O
TS27: bronchus	Present		4O

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Present	Regionally restricted	5A	Expression was exclusively in the budding dental epithelium with a preference for the buccal edge.
TS21: tooth mesenchyme	Absent		5A

Structure	Level	Pattern	Image	Note
TS16: limb mesenchyme	Present		6A
TS16: surface ectoderm	Present		6A
TS16: apical ectodermal ridge	Present	Regionally restricted	6A	Expression was strong in the outermost cells of the apical ectodermal ridge and weaker in the rest of the structure.

Structure	Level	Pattern	Image	Note
TS20: limb pre-cartilage condensation	Present

Structure	Level	Pattern	Image	Note
TS23: limb mesenchyme	Present	Regionally restricted		Expression was detected in differentiating chondrocytes in the precartilage mesenchymal condensations.

Structure	Level	Pattern	Image	Note
TS26: femur	Present	Regionally restricted	6I	Expression was detected in mature chondrocytes, with virtually no expression in the proliferating zone or in the future resting zone.

Structure	Level	Pattern	Image	Note
TS26: chondrocranium	Present	Regionally restricted		Expression was in the osteoblasts and osteoclast. Expression was in the suture mesenchyme.