Antibody:	Anti-AZ
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS12: conceptus	Present

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Present		1F	High levels of expression were noted in fibers emanating from the trigeminal ganglion, and weaker levels in the somata of the neurons.

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present
TS17: spinal nerve	Present

Structure	Level	Pattern	Image	Note
TS18: latero-nasal process	Strong
TS18: medial-nasal process	Strong
TS18: central nervous system	Present	Regionally restricted		Expression was detected in the mantle layer, while the ventricular and ependymal layers were devoid of expression.
TS18: liver	Weak
TS18: cranial nerve	Present	Regionally restricted		Expression was detected in the infraorbital nerve, a maxillary branch from the trigeminal ganglion.
TS18: embryo mesenchyme	Present
TS18: head mesenchyme	Present
TS18: surface ectoderm	Present

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Present
TS21: dorsal root ganglion	Present
TS21: neocortex	Present
TS21: spinal cord mantle layer	Present
TS21: thalamus	Present

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted		Expression was detected in the cell processes.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Regionally restricted		Expression was in motor neurons.
TS23: brain	Present
TS23: lens fiber	Present
TS23: eyelid	Strong	Regionally restricted		Expression was detected in the margins of the eyelids.

Structure	Level	Pattern	Image	Note
TS26: cranial ganglion	Present
TS26: dorsal root ganglion	Present

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		1G , 1I

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	1H	Intense nuclear localization was noted in migratory cells.
TS26: cerebellum lateral part marginal layer	Absent		1H	Cells of the external germinal layer at the pial surface were essentially devoid of staining.

Structure	Level	Pattern	Image	Note
TS27: cranial ganglion	Present
TS27: cerebellum external granule cell layer	Present	Regionally restricted		Expression was detected in a subset of cells.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		1N
TS28: hippocampus	Present		1N

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Non-Uniform	1O	Some cells were labeled.
TS28: cerebellum Purkinje cell layer	Present		1O
TS28: cerebellum molecular layer	Present	Regionally restricted	1O	Expression was in basket and stellate cells, but not in Purkinje cell dendrites.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	1Q
TS28: dentate gyrus	Present		1Q
TS28: hippocampus CA1	Present		1Q	Expression was detected in the neurons.
TS28: hippocampus CA2	Present		1Q	Expression was detected in the neurons.
TS28: hippocampus CA3	Present		1Q	Expression was detected in the neurons.

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present
TS28: cerebral cortex	Present

Structure	Level	Pattern	Image	Note
TS21: lens fiber	Present	Regionally restricted	2B	Expression was detected in primary lens fiber cells.
TS21: cornea	Weak		2B
TS21: lens epithelium	Weak		2B
TS21: pigmented retinal epithelium	Weak		2B
TS21: retina	Weak		2B

Structure	Level	Pattern	Image	Note
TS28: lens fiber	Present	Regionally restricted		Expression was detected in the secondary lens fibers.
TS28: neural retina	Present
TS28: pigmented retinal epithelium	Present
TS28: ciliary body	Present			Expression was detected in the ciliary process.

Structure	Level	Pattern	Image	Note
TS23: liver	Present	Single cells

Structure	Level	Pattern	Image	Note
TS26: liver	Weak
TS26: gut	Present	Graded		Expression exhibited a proximo-distal gradient; in some distal segments, expression was barely detectable.
TS26: brown fat	Present

Structure	Level	Pattern	Image	Note
TS27: brown fat	Present

Structure	Level	Pattern	Image	Note
TS28: brown fat	Present

Structure	Level	Pattern	Image	Note
TS22: gut	Present		3A	Expression was detected in the gut endoderm.
TS22: intestine smooth muscle	Present		3A
TS22: gut mesenchyme	Weak		3A

Structure	Level	Pattern	Image	Note
TS26: intestinal villus	Present	Regionally restricted	3D	Expression was weak in the proliferating basal cells of the villi, but was strong in the more differentiated cells of the villi.
TS26: enteric ganglion	Strong		3D

Structure	Level	Pattern	Image	Note
TS26: small intestine	Present	Regionally restricted	3G	Expression was weak in the proliferating basal cells of the villi, but was strong in the more differentiated cells of the villi.

Structure	Level	Pattern	Image	Note
TS26: renal tubule	Present		3J
TS26: nephrogenic zone	Absent		3J

Structure	Level	Pattern	Image	Note
TS28: renal corpuscle	Absent		3K
TS28: renal proximal convoluted tubule	Present		3K
TS28: renal tubule	Strong		3K

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Strong	Regionally restricted	4E	In sections through the maxillary process the surface ectoderm had strong levels of staining except in pre-germ hair follicles (stage 0), which were detected by a local decrease in the immunostaining.
TS19: head mesenchyme	Present	Regionally restricted	4E	Expression was detected in the dermal papilla of stage 1 hair germ.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Strong	Regionally restricted	4F	Expression was strong in the surface ectoderm, except in the stage 2 vibrissa where it was decreased.
TS19: head mesenchyme	Weak	Regionally restricted	4F	Expression was weak in dermal papilla cells.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	4H	In stage 5 vibrissae, expression was strong in precortex cells and outer root sheath. The hair matrix was devoid of expression.
TS22: vibrissa dermal component	Present		4H

Structure	Level	Pattern	Image	Note
TS26: vibrissa dermal component	Strong	Regionally restricted	4I	Strong expression was detected in the dermal sheath around the vibrissae follicles.

Structure	Level	Pattern	Image	Note
TS19: lung	Present	Regionally restricted	4K	Expression was detected in the lung endoderm.
TS19: lung epithelium	Present		4K
TS19: lung mesenchyme	Present		4K

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present		5D
TS21: oral epithelium	Present	Regionally restricted		Expression was detected in a subset of cells extending from the oral surface epithelium to the central contingent of the budding dental epithelium.
TS21: tooth epithelium	Present	Regionally restricted		Expression was detected in a subset of cells extending from the oral surface epithelium to the central contingent of the budding dental epithelium.

Structure	Level	Pattern	Image	Note
TS16: limb ectoderm	Present		6B
TS16: limb mesenchyme	Present		6B
TS16: surface ectoderm	Present		6B

Structure	Level	Pattern	Image	Note
TS20: limb interdigital region	Strong		6E
TS20: limb pre-cartilage condensation	Strong		6E

Structure	Level	Pattern	Image	Note
TS23: limb pre-cartilage condensation	Present		6F	Expression was detected in chondrocytes.
TS23: limb interdigital region mesenchyme	Present		6F	Expression was detected in preapoptotic mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: limb pre-cartilage condensation	Present		6G	Expression was detected in chondrocytes.

Structure	Level	Pattern	Image	Note
TS26: femur	Present	Regionally restricted	6J	Expression was detected in mature chondrocytes, with virtually no expression in the proliferating zone or in the future resting zone.

Structure	Level	Pattern	Image	Note
TS26: chondrocranium	Present	Regionally restricted		Expression was in the osteoblasts and osteoclast.
TS26: suture	Present	Regionally restricted		Expression was not in the suture mesenchyme, except in a subset of cells with fragmented nuclei.