Probe:	CHL probe1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: somite	Trace			Expression barely detectable over somites.
TS15: limb	Absent

Structure	Level	Pattern	Image	Note
TS17: somite	Strong	Regionally restricted	7A/B
TS17: limb mesenchyme	Present	Diffuse	7A/B

Structure	Level	Pattern	Image	Note
TS17: dermatome	Strong		7C/D

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted		Expression in differentiating fields of ventral telencephalon destined to become olfactory bulb.
TS17: hindbrain	Present	Regionally restricted		Expression in differentiating fields of dorsal rhombencephalon, destined to become cerebellum.

Structure	Level	Pattern	Image	Note
TS23: forelimb phalanx	Strong	Regionally restricted	7E/F	Expression in hypertrophic chondrocytes in neural arch, radius, metacarpal and phalangeal bones.
TS23: metacarpus	Strong	Regionally restricted	7E/F	Expression in hypertrophic chondrocytes in neural arch, radius, metacarpal and phalangeal bones.
TS23: radius	Strong	Regionally restricted	7E/F	Expression in hypertrophic chondrocytes in neural arch, radius, metacarpal and phalangeal bones.
TS23: vertebra	Strong	Regionally restricted	7E/F	Expression in hypertrophic chondrocytes in neural arch, radius, metacarpal and phalangeal bones.
TS23: limb mesenchyme	Present	Regionally restricted	7E/F	Expression in areas of condensed mesenchyme adjacent to developing bones and in subcutaneous stromal cells.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	7G/H	Expression in areas of mesenchymal cells where precartilaginous condensations begin to form.

Structure	Level	Pattern	Image	Note
TS19: pre-cartilage condensation	Present			Expression in mesenchymal cells concurrent with formation of precartilaginous condensations.

Structure	Level	Pattern	Image	Note
TS20: chondrocranium	Present	Regionally restricted		Expression in cephalic mesenchyme in areas where basioccipital and exoccipital bones form.
TS20: head mesenchyme	Present	Regionally restricted		Expression in cephalic mesenchyme in areas where basioccipital and exoccipital bones form.
TS20: mandible primordium	Present			Expression mesenchymal cells in mandible.
TS20: vertebral pre-cartilage condensation	Present			Expression adjacent to dorsal root ganglia in area where vertebra form.

Structure	Level	Pattern	Image	Note
TS21: radius cartilage condensation	Present	Regionally restricted	7I/J	Expression in hypertrophic chondrocytes in radius.
TS21: forelimb joint	Present	Regionally restricted	7I/J	Expression in perichondrial mesemchyme adjacent to elbow and wrist joints.

Structure	Level	Pattern	Image	Note
TS21: clavicle pre-cartilage condensation	Present
TS21: thoracic segment skeleton	Present	Regionally restricted		Expression in clavicle and sternum.

Structure	Level	Pattern	Image	Note
TS23: hindlimb phalanx	Strong	Regionally restricted	7K/L	Expression in hypertrophic chondrocytes of radius, tarsals, metatarsals and phalanges.
TS23: metatarsus mesenchyme	Strong	Regionally restricted	7K/L	Expression in hypertrophic chondrocytes of radius, tarsals, metatarsals and phalanges.
TS23: radius	Strong	Regionally restricted	7K/L	Expression in hypertrophic chondrocytes of radius, tarsals, metatarsals and phalanges.
TS23: tarsus	Strong	Regionally restricted	7K/L	Expression in hypertrophic chondrocytes of radius, tarsals, metatarsals and phalanges.
TS23: perichondrium	Weak	Regionally restricted	7K/L	Expression declines in perichondrial mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS26: long bone epiphysis	Weak	Regionally restricted	7N/O	Expression present at diminished levels, restricted to hypertrophic chondrocytes at epiphyseal growth plates.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate hypertrophic zone	Weak		8A	Expression in hypertrophic chondrocytes in epiphyseal growth plate region of femur.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	8B	Expression in a population of bone marrow stromal cells.

Structure	Level	Pattern	Image	Note
TS28: duodenum	Present	Regionally restricted	8C/D	Expression in layer of stromal/connective tissue cells between submucosa and muscularis.

Structure	Level	Pattern	Image	Note
TS28: connective tissue	Present	Regionally restricted		Expression in subdermal fusiform stromal cells, connective tissue cells of the epimysium and perimysium surrounding bundles of skeletal muscle, and in perivascular cells.

Structure	Level	Pattern	Image	Note
TS28: outer renal medulla	Strong		8E	Expression primarily in outer medulla and medullary rays.
TS28: renal cortex	Present	Regionally restricted	8E	Expression primarily in outer medulla and medullary rays.

Structure	Level	Pattern	Image	Note
TS28: renal tubule	Present	Regionally restricted	8F	Expression localized in segments of thick ascending tubules.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		8G/H

Structure	Level	Pattern	Image	Note
TS28: lymph node	Present	Regionally restricted		Expression in trabecular connective tissue cells in lymph nodes.`
TS28: lung connective tissue	Present	Regionally restricted		Expression in perivascular cells in the lung.
TS28: uterus	Present	Regionally restricted		Expression in myofibroblasts in the uterus.
TS28: white fat	Present	Regionally restricted		Expression in stromal cells in white adipose tissue.
TS28: main olfactory bulb	Present	Regionally restricted		Expression in olfactory bulb neurons
TS28: cerebral cortex	Present	Regionally restricted		Expression in cerebral cortical neurons and glial-like cells.