Ghrl RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:156957 Hill JT, et al., Nkx2.2 activates the ghrelin promoter in pancreatic islet cells. Mol Endocrinol. 2010 Feb;24(2):381-90
Assay type:	RT-PCR
MGI Accession ID:	MGI:5642286
Gene symbol:	Ghrl
Gene name:	ghrelin

Probe:	Long-pF, Long-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Real time PCR was used for these studies; cyclophilin B was used as the loading control. The primer set in this experiment used amplifies the long form of the transcript, which includes the first exon.

Results

Image: 1C long transcript

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
Wildtype	E18.5	TS26: pancreas	Present (a)	Not Specified; total RNA	Not Specified		Not Specified
Nkx2.2 KO	E18.5	TS26: pancreas	Present (b)	Not Specified; total RNA	involves: 129X1/SvJ	Nkx2-2^tm1Jlr/Nkx2-2^tm1Jlr	Not Specified

Notes:
(a) This form represents 26.8% of all transcripts for this gene at this time.
(b) Expression levels of all transcript forms are increased in the mutant relative to wild type. In addition, this form represents 37.9% of all transcripts for this gene at this time, an increase in its proportion compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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