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Gene Expression Data
Assay Details
Assay
Reference: J:210262 Boucher E, et al., Ontogeny of adrenal-like glucocorticoid synthesis pathway and of 20alpha-hydroxysteroid dehydrogenase in the mouse lung. BMC Res Notes. 2014;7:119
Assay type: RT-PCR
MGI Accession ID: MGI:5641685
Gene symbol: Cyp21a1
Gene name: cytochrome P450, family 21, subfamily a, polypeptide 1
Probe: 21-hydroxylase-pA, 21-hydroxylase-pB
Assay notes: Expression was analyzed using qRT-PCR. Relative expression levels were calculated with a standard curve using serial dilutions and normalized to reference gene expression. The mean value obtained on GD 19.5 was fixed as onefold.
Results
Image: 2E ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
1 Males E15.5 TS23: lung Present (b) Not Specified; total RNA Not Specified Male
1 Females E15.5 TS23: lung Present (b) Not Specified; total RNA Not Specified Female
2 Males E15.5 TS23: lung Present (b) Not Specified; total RNA Not Specified Male
2 Females E15.5 TS23: lung Present (b) Not Specified; total RNA Not Specified Female
3 Males E15.5 TS23: lung Present (b) Not Specified; total RNA Not Specified Male
3 Females E15.5 TS23: lung Present (b) Not Specified; total RNA Not Specified Female
4 Males E19.5 TS26: lung Present (b) Not Specified; total RNA Not Specified Male
4 Females E19.5 TS26: lung Present (b) Not Specified; total RNA Not Specified Female
5 Males E19.5 TS26: lung Present (b) Not Specified; total RNA Not Specified Male
5 Females E19.5 TS26: lung Present (b) Not Specified; total RNA Not Specified Female
6 Males E19.5 TS26: lung Present (b) Not Specified; total RNA Not Specified Male
6 Females E19.5 TS26: lung Present (b) Not Specified; total RNA Not Specified Female
7 Males P0 TS27: lung Present (b) Not Specified; total RNA Not Specified Male
7 Females P0 TS27: lung Present (b) Not Specified; total RNA Not Specified Female
8 Males P0 TS27: lung Present (b) Not Specified; total RNA Not Specified Male
8 Females P0 TS27: lung Present (b) Not Specified; total RNA Not Specified Female
9 Males P0 TS27: lung Present (b) Not Specified; total RNA Not Specified Male
9 Females P0 TS27: lung Present (b) Not Specified; total RNA Not Specified Female
10 Males P3 TS27: lung Present (b) Not Specified; total RNA Not Specified Male
10 Females P3 TS27: lung Present (b) Not Specified; total RNA Not Specified Female
11 Males P3 TS27: lung Present (b) Not Specified; total RNA Not Specified Male
11 Females P3 TS27: lung Present Not Specified; total RNA Not Specified Female
12 Males P3 TS27: lung Present (b) Not Specified; total RNA Not Specified Male
12 Femaels P3 TS27: lung Present (b) Not Specified; total RNA Not Specified Female
13 Males P7 TS28: lung Present (c) Not Specified; total RNA Not Specified Male
13 Females P7 TS28: lung Present (c) Not Specified; total RNA Not Specified Female
14 Males P7 TS28: lung Present (c) Not Specified; total RNA Not Specified Male
14 Females P7 TS28: lung Present (c) Not Specified; total RNA Not Specified Female
15 Males P7 TS28: lung Present (c) Not Specified; total RNA Not Specified Male
15 Females P7 TS28: lung Present (c) Not Specified; total RNA Not Specified Female
16 Males P15 TS28: lung Present (d) Not Specified; total RNA Not Specified Male
16 Females P15 TS28: lung Present (d) Not Specified; total RNA Not Specified Female
17 Males P15 TS28: lung Present (d) Not Specified; total RNA Not Specified Male
17 Females P15 TS28: lung Present (d) Not Specified; total RNA Not Specified Female
18 Males P15 TS28: lung Present (d) Not Specified; total RNA Not Specified Male
18 Females P15 TS28: lung Present (d) Not Specified; total RNA Not Specified Female
Notes:
(a) Authors report a significant influence of age on expression, but not of sex or sex*age.
(b) Expression levels stable from E15.5 to PN 3.
(c) 4.3-fold increase in expression from PN 3 to PN 7
(d) Expression levels stable from PN 7 to PN 15.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory