About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:191315 Courtois A, et al., The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development. J Cell Biol. 2012 Aug 6;198(3):357-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5641391
Gene symbol: Pcnt
Gene name: pericentrin (kendrin)
Antibody: Anti-Pericentrin
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: It is possible that a different antibody was used for this assay MGI:3806286.
Results
Specimen 1A: postnatal adult (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 1A Expression is in microtubule organizing centers.
Specimen 1B: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1B Expression is in microtubule organizing centers.
Specimen 1C: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1C Expression is in microtubule organizing centers.
Specimen 1D: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1D Expression is in microtubule organizing centers.
Specimen 1E: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1E Expression is in microtubule organizing centers.
Specimen 1F: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1F Expression is in microtubule organizing centers.
Specimen 1G: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1G Expression is in microtubule organizing centers.
Specimen 1H: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1H Expression is in microtubule organizing centers.
Specimen 1I: embryonic day 0.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 1I Expression is in microtubule organizing centers.
Specimen 3C left: embryonic day 2.0 (more )
Note: 2-cell to 4-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS3: 4-cell stage conceptus Present 3C left Expression is in microtubule organizing centers.
Specimen 3C middle: embryonic day 2.0 (more )
Note: 2-cell to 4-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS3: 4-cell stage conceptus Present 3C middle Expression is in microtubule organizing centers.
Specimen 3C right: embryonic day 2.0 (more )
Note: 2-cell to 4-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS3: 4-cell stage conceptus Present 3C right Expression is in microtubule organizing centers.
Specimen 3D left: embryonic day 3.0 (more )
Note: 8-cell to 16-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D left
Specimen 3D middle: embryonic day 3.0 (more )
Note: 8-cell to 16-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D middle Expression is in microtubule organizing centers.
Specimen 3D right: embryonic day 3.0 (more )
Note: 8-cell to 16-cell. Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D right Expression is in microtubule organizing centers.
Specimen 3E left: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS6: embryo Present 3E left Expression is in microtubule organizing centers.
Specimen 3E middle: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS6: embryo Present 3E middle Expression is in microtubule organizing centers.
Specimen 3E right: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- microtubules. Fixative was 2% formaldehyde, 0.05% glutaraldehyde.
Structure Level Pattern Image Note
TS6: embryo Present 3E right Expression is in microtubule organizing centers.
Specimen 4A 16-cell stage: embryonic day 3.0 (more )
Note: Double labeled: red - Pcnt; green- Cetn1 (assay MGI:5641394). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS4: inner cell mass Present 4A pericentrin 16-cell stage , 4A merge 16-cell stage Expression is in microtubule organizing centers.
Specimen 4A E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pcnt; green- Cetn1 (assay MGI:5641394). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS5: inner cell mass Present 4A merge E3.5 , 4A pericentrin E3.5 Expression is in microtubule organizing centers.
Specimen 4A E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- Cetn1 (assay MGI:5641394). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS6: embryo Present 4A merge E4.5 , 4A pericentrin E4.5 Expression is in microtubule organizing centers.
Specimen 4B left 16-cell: embryonic day 3.0 (more )
Note: Double labeled: red - Pcnt; green- Ccp110 (assay MGI:5641395). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B left pericentrin 16-cell stage , 4B left merge 16-cell stage Expression is in microtubule organizing centers.
Specimen 4B left E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pcnt; green- Ccp110 (assay MGI:5641395). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS5: inner cell mass Present 4B left pericentrin E3.5 , 4B left merge E3.5 Expression is in microtubule organizing centers.
Specimen 4B left E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green- Ccp110 (assay MGI:5641395). Fixative was 100% methanol.
Structure Level Pattern Image Note
TS6: embryo Present 4B left pericentrin E4.5 , 4B left merge E4.5 Expression is in microtubule organizing centers.
Specimen 4B right 16-cell: embryonic day 3.0 (more )
Note: Triple labeled: magenta - Pcnt; green - Kif12 (assay MGI:5641396); red - microtubules. Fixative was 100% methanol.
Structure Level Pattern Image Note
TS4: inner cell mass Present 4B right pericentrin 16-cell stage , 4B right merge 16-cell stage Expression is in microtubule organizing centers.
Specimen 4B right E3.5: embryonic day 3.5 (more )
Note: Triple labeled: magenta - Pcnt; green - Kif12 (assay MGI:5641396); red - microtubules.
Structure Level Pattern Image Note
TS5: inner cell mass Present 4B right pericentrin E3.5 , 4B right merge E3.5 Expression is in microtubule organizing centers.
Specimen 4B right E4.5: embryonic day 4.5 (more )
Note: Triple labeled: magenta - Pcnt; green - Kif12 (assay MGI:5641396); red - microtubules.
Structure Level Pattern Image Note
TS6: embryo Present 4B right merge E4.5 , 4B right pericentrin E4.5 Expression is in microtubule organizing centers.
Specimen S3: embryonic day 2.0 (more )
Note: Double labeled: red - Pcnt; green- microtubules.
Structure Level Pattern Image Note
TS3: 8-cell stage conceptus Present S3 Expression is in microtubule organizing centers.
Specimen S4 16-cell stage: embryonic day 3.0 (more )
Note: Double labeled: red - Pcnt; green - Odf2 (assay MGI:5641400).
Structure Level Pattern Image Note
TS4: inner cell mass Present S4_pericentrin 16-cell stage Expression is in microtubule organizing centers.
Specimen S4 E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pcnt; green - Odf2 (assay MGI:5641400).
Structure Level Pattern Image Note
TS5: inner cell mass Present S4_pericentrin E3.5 Expression is in microtubule organizing centers.
Specimen S4 E4.5: embryonic day 4.5 (more )
Note: Double labeled: red - Pcnt; green - Odf2 (assay MGI:5641400).
Structure Level Pattern Image Note
TS6: embryo Present S4_pericentrin E4.5 Expression is in microtubule organizing centers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory