Tg(Ckm-SLC2A4/EGFP)1Samc
Transgene Detail
|
|
| Symbol: |
Tg(Ckm-SLC2A4/EGFP)1Samc |
| Name: |
transgene insertion 1, Samuel Cushman |
| MGI ID: |
MGI:5641334 |
| Synonyms: |
HA-GLUT4-GFP |
| Transgene: |
Tg(Ckm-SLC2A4/EGFP)1Samc Location: unknown
|
| Alliance: |
Tg(Ckm-SLC2A4/EGFP)1Samc page
|
|
|
|
| Transgene Type: |
|
Transgenic (Humanized sequence, Inserted expressed sequence, Reporter) |
| Mutation: |
|
Insertion
|
| |
|
Tg(Ckm-SLC2A4/EGFP)1Samc expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
SLC2A4 (6517) |
|
|
|
| |
|
Mutation details: The transgenic construct consists of a hemaglutinin (HA) epitope and the human SLC2A4 (GLUT4) COOH terminus fused to an intracellular EGFP tag under the control of a muscle creatinine kinase promoter, enhancer 1, untranslated exon 1, 3kb of intron 1 (including the enhancer 2 region), and the first 16 bp of exon 2 and a poly(A) signal. The HA epitope tag is in the first extracellular loop that is exposed on the cell surface. Western blot analysis confirmed expression in skeletal muscle (soleus, gastrocnemius, extensor digitorum longus, flexor digitorum breviis). Expression is 2.2 fold higher than the endogenous protein.
(J:184630)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
| Original: |
J:184630 Lizunov VA, et al., Insulin stimulates fusion, but not tethering, of GLUT4 vesicles in skeletal muscle of HA-GLUT4-GFP transgenic mice. Am J Physiol Endocrinol Metab. 2012 Apr;302(8):E950-60 |
| All: |
2 reference(s) |
|
Analysis Tools
