Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Ckm-SLC2A4/EGFP)1Samc
Name:	transgene insertion 1, Samuel Cushman
MGI ID:	MGI:5641334
Synonyms:	HA-GLUT4-GFP
Transgene:	Tg(Ckm-SLC2A4/EGFP)1Samc  Location: unknown
Alliance:	Tg(Ckm-SLC2A4/EGFP)1Samc page

Strain of Origin:

FVB/N

Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence, Reporter) Mutation:    Insertion   Tg(Ckm-SLC2A4/EGFP)1Samc expresses 1 gene Transgene expresses: Organism Expressed Gene Homolog in Mouse Note human SLC2A4 (6517) Slc2a4  (MGI:95758)   Mutation details: The transgenic construct consists of a hemaglutinin (HA) epitope and the human SLC2A4 (GLUT4) COOH terminus fused to an intracellular EGFP tag under the control of a muscle creatinine kinase promoter, enhancer 1, untranslated exon 1, 3kb of intron 1 (including the enhancer 2 region), and the first 16 bp of exon 2 and a poly(A) signal. The HA epitope tag is in the first extracellular loop that is exposed on the cell surface. Western blot analysis confirmed expression in skeletal muscle (soleus, gastrocnemius, extensor digitorum longus, flexor digitorum breviis). Expression is 2.2 fold higher than the endogenous protein. (J:184630)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:184630 Lizunov VA, et al., Insulin stimulates fusion, but not tethering, of GLUT4 vesicles in skeletal muscle of HA-GLUT4-GFP transgenic mice. Am J Physiol Endocrinol Metab. 2012 Apr;302(8):E950-60
All:	1 reference(s)