Gja1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:72502 Perez-Armendariz EM, et al., Developmental regulation of connexin 43 expression in fetal mouse testicular cells. Anat Rec. 2001 Nov 1;264(3):237-46
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5640780
Gene symbol:	Gja1
Gene name:	gap junction protein, alpha 1

Antibody:	anti-Connexin 43
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Fixative was 70% ethanol.

Results

Specimen 1A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Uniform	1A
TS19: mesonephric tubule	Present	Punctate	1A

Specimen 1B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: testis	Present	Uniform	1B

Specimen 2A/B: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: interstitium of the testis	Present	Regionally restricted	2A/B	Expression is in spindle-shaped interstitial cell clumps that surround the seminiferous cords.

Specimen 2C/D: embryonic day 14.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS22: interstitium of the testis		Present	Regionally restricted	2C/D	Expression is in spindle-shaped interstitial cell clumps that surround the seminiferous cords.
TS22: Leydig cell	Leydig cell	Present		2C/D

Specimen 2E/F: embryonic day 16.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: interstitium of the testis		Present	Regionally restricted	2E/F	Expression is in cell aggregates that surround the seminiferous cords.
TS24: Leydig cell	Leydig cell	Present		2E/F

Specimen 2G/H: embryonic day 18.5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: interstitium of the testis		Present	Regionally restricted	2G/H	Expression is in cell aggregates that surround the seminiferous cords.
TS26: Leydig cell	Leydig cell	Present		2G/H

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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