Gene Expression Data
Assay Details
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Assay
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Reference:
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J:172381
Osterberg N, et al., Sim1 is a novel regulator in the differentiation of mouse dorsal raphe serotonergic neurons. PLoS One. 2011;6(4):e19239
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5640619
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Gene symbol:
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Th
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Gene name:
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tyrosine hydroxylase
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Antibody:
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anti-TH
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
1B:
postnatal newborn
(more  )
Note: (C) is a higher magnification of the boxed area in (A). Double labeled: blue - Th; brown - Sim1 (assay MGI:5640623).
Specimen
1B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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(C) is a higher magnification of the boxed area in (A). Double labeled: blue - Th; brown - Sim1 (assay MGI:5640623).
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Specimen
not shown wt:
embryonic day 14.5
(more )
Specimen
not shown wt:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown Sim1-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Sim1tm1Fan/Sim1tm1Fan
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: midbrain |
Present |
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The authors state that there are no significant differences in the number of Th-positive neurons in this mutant compared to wild-type.
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Specimen
not shown P0 wt:
postnatal day 0
(more )
Specimen
not shown P0 wt:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
not shown P0 -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Sim1tm1Fan/Sim1tm1Fan
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: substantia nigra |
Present |
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The authors state that there are no significant differences in the number of Th-positive neurons in this mutant compared to wild-type.
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| TS27: ventral tegmental area |
Present |
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The authors state that there are no significant differences in the number of Th-positive neurons in this mutant compared to wild-type.
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Analysis Tools