Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS23: esophagus epithelium	Moderate		1a
TS23: esophagus mesenchyme	Absent		1a

Structure	Level	Pattern	Image	Note
TS25: esophagus epithelium	Present	Widespread	1b	Expressed throughout most of the length, increased from moderate levels in the region of the lower esophageal sphincter to high levels in more antelior regions.
TS25: esophagus mesenchyme	Absent		1b

Structure	Level	Pattern	Image	Note
TS28: esophagus epithelium	Strong	Widespread	1c	High level expression in most of the regions.
TS28: esophagus lamina propria	Absent		1c
TS28: esophagus muscularis	Absent		1c
TS28: esophagus muscularis mucosa	Absent		1c
TS28: esophagus serosa	Absent		1c
TS28: esophagus submucosa	Absent		1c

Structure	Level	Pattern	Image	Note
TS28: esophagus epithelium	Strong	Widespread	1d
TS28: stomach proventricular region	Present	Regionally restricted	1d	Expressed in the circular muscle of the forestomach extended into the lower esophageal sphincter.
TS28: stomach smooth muscle	Present	Regionally restricted	1d	Expressed in the circular muscle of the forestomach extended into the lower esophageal sphincter.
TS28: esophagus lamina propria	Absent		1d
TS28: esophagus muscularis	Absent		1d
TS28: esophagus muscularis mucosa	Absent		1d
TS28: esophagus serosa	Absent		1d
TS28: esophagus submucosa	Absent		1d

Structure	Level	Pattern	Image	Note
TS20: stomach mesenchyme	Present	Widespread	3b , 3a	Expressed in the entire thickness of this mesenchyme but was stronger in the anterior portion of the caudal stomach region.
TS20: enteric nervous system	Absent		3a , 3b
TS20: stomach epithelium	Absent		3a , 3b
TS20: liver	Present	Regionally restricted	3a , 3b	Expressed in the smooth muscle of blood vessels that supplied one of the liver lobes adjacent to the stomach. Also expressed in a thin layer at the edge of these liver lobes.

Structure	Level	Pattern	Image	Note
TS21: stomach mesenchyme	Present	Widespread	3c , 3d	Expressed in those layers that develop into the lamina propria and circular and longitudinal smooth muscle.
TS21: enteric nervous system	Absent		3c , 3d
TS21: stomach epithelium	Absent		3c , 3d

Structure	Level	Pattern	Image	Note
TS23: stomach mesenchyme	Strong	Regionally restricted	3f , 3e	Expressed in the longitudinal and circular smooth muscle layers with the strongest expression in the antrum occurring on its anterior side.
TS23: enteric nervous system	Absent		3f , 3e
TS23: stomach epithelium	Absent		3f , 3e

Structure	Level	Pattern	Image	Note
TS23: pyloric antrum	Strong	Regionally restricted	4b , 4a	Expressed in the longitudinal and circular smooth muscle layers with the strongest expression in the antrum occurring on its anterior side. Weaker expression in the lamina propria of the mucosa.
TS23: stomach pyloric region mesenchyme	Strong	Regionally restricted	4b , 4a	Expressed in the longitudinal and circular smooth muscle layers with the strongest expression in the antrum occurring on its anterior side. Weaker expression in the lamina propria of the mucosa.
TS23: duodenum rostral part mesenchyme	Present	Regionally restricted	4b , 4a	Expressed in the circular muscle layer.
TS23: stomach mesenchyme	Present	Regionally restricted	4b , 4a	Weaker expression in the lamina propria of the mucosa of the antrum and corpus.
TS23: enteric nervous system	Absent		4b , 4a
TS23: stomach epithelium	Absent		4b , 4a

Structure	Level	Pattern	Image	Note
TS25: pyloric antrum	Strong	Regionally restricted	4c , 4d	Expressed in the longitudinal and circular smooth muscle layers with the strongest expression in the antrum occurring on its anterior side. Slightly increased expression in the lamina propria of the mucosa.
TS25: stomach pyloric region mesenchyme	Strong	Regionally restricted	4c , 4d	Expressed in the longitudinal and circular smooth muscle layers with the strongest expression in the antrum occurring on its anterior side. Slightly increased expression in the lamina propria of the mucosa.
TS25: duodenum mesenchyme	Present	Regionally restricted	4d , 4c	Expressed in the circular muscle layer.
TS25: enteric nervous system	Absent		4d , 4c
TS25: stomach epithelium	Absent		4d , 4c

Structure	Level	Pattern	Image	Note
TS28: stomach proventricular region	Present	Regionally restricted	5a	Expressed in the circular layer of the muscle wall only.
TS28: intrinsic nerve plexus	Absent		5a
TS28: stomach epithelium	Absent		5a
TS28: stomach submucosa	Absent		5a
TS28: stomach mucosa	Present	Regionally restricted	5a	Expressed in the lamina propria of the mucosa of the antrum and corpus.
TS28: stomach smooth muscle	Present	Regionally restricted	5a	Expressed in the circular and longitudinal muscle layers of the antrum and some regions of the corpus and only in the circular layer of the remaining regions of the corpus.

Structure	Level	Pattern	Image	Note
TS28: pyloric antrum	Present	Regionally restricted	5c , 5b	Expressed in the circular and longitudinal muscle layers.
TS28: stomach smooth muscle	Present	Regionally restricted	5c , 5b	Expressed in the circular and longitudinal muscle layers.
TS28: blood vessel smooth muscle	Present		5c , 5b
TS28: intrinsic nerve plexus	Absent		5b , 5c
TS28: stomach epithelium	Absent		5b , 5c
TS28: stomach submucosa	Absent		5b , 5c

Structure	Level	Pattern	Image	Note
TS28: stomach proventricular region	Present	Regionally restricted	5e , 5d	Only expressed in the circular muscle.
TS28: intrinsic nerve plexus	Absent		5e , 5d
TS28: stomach epithelium	Absent		5e , 5d
TS28: stomach submucosa	Absent		5e , 5d

Structure	Level	Pattern	Image	Note
TS20: intestine mesenchyme	Present	Diffuse	6a , 6b	Expressed throughout the large intestine mesenchyme.
TS20: enteric nervous system	Absent		6b , 6a
TS20: intestine epithelium	Absent		6b , 6a

Structure	Level	Pattern	Image	Note
TS20: intestine mesenchyme	Present	Diffuse	6d , 6c	Expressed throughout the large intestine mesenchyme.
TS20: enteric nervous system	Absent		6c , 6d
TS20: intestine epithelium	Absent		6c , 6d

Structure	Level	Pattern	Image	Note
TS21: intestine muscularis	Present	Regionally restricted	6e , 6f	Expressed in the developing external muscle layers.
TS21: small intestine	Present	Regionally restricted	6e , 6f	Expressed in the developing external muscle layers.
TS21: intestine mesenchyme	Present	Regionally restricted	6f , 6e	Mainly expressed in the muscle wall; weak expression in the remaining mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: intestine muscularis	Present	Regionally restricted	7b , 7a	Expressed in the circular muscle layer.
TS23: small intestine	Present	Regionally restricted	7b , 7a	Expressed in the circular muscle layer.

Structure	Level	Pattern	Image	Note
TS25: intestine muscularis	Present	Regionally restricted	7c , 7d	Expressed in the circular muscle layer.
TS25: small intestine	Present	Regionally restricted	7c , 7d	Expressed in the circular muscle layer.

Structure	Level	Pattern	Image	Note
TS28: small intestine smooth muscle	Present	Regionally restricted	8b , 8a , 8c	Reduced expression in the circular muscle layer.
TS28: blood vessel	Present		8a , 8b , 8c
TS28: intrinsic nerve plexus	Absent		8c , 8a , 8b
TS28: small intestine mucosa	Absent		8c , 8a , 8b
TS28: blood vessel smooth muscle	Present	Regionally restricted	8a , 8b , 8c	Expressed in blood vessels that exited the mesentery to supply the intestines.

Structure	Level	Pattern	Image	Note
TS23: intestine muscularis	Present	Regionally restricted	9a , 9b	Expressed mostly in the circular muscle layer of the cecum/large intestine.

Structure	Level	Pattern	Image	Note
TS25: intestine muscularis	Present	Regionally restricted	9c , 9d	Expressed mostly in the circular muscle layer of the cecum/large intestine.
TS25: large intestine	Present	Regionally restricted	9c , 9d	Expressed mostly in the circular muscle layer of the cecum/large intestine.
TS25: blood vessel	Present		9c , 9d

Structure	Level	Pattern	Image	Note
TS25: intestine muscularis	Strong	Regionally restricted	10a , 10b	Strong expression in the circular smooth muscle layer, and weaker in or absent from various anterior-posterior levels of the longitudinal smooth muscle layer.
TS25: large intestine	Strong	Regionally restricted	10a , 10b	Strong expression in the circular smooth muscle layer, and weaker in or absent from various anterior-posterior levels of the longitudinal smooth muscle layer.
TS25: enteric nervous system	Absent		10a , 10b
TS25: intestine epithelium	Absent		10a , 10b

Structure	Level	Pattern	Image	Note
TS25: intestine muscularis	Strong	Regionally restricted	10c , 10d	Strong expression in the circular smooth muscle layer, and weaker in or absent from various anterior-posterior levels of the longitudinal smooth muscle layer.
TS25: large intestine	Strong	Regionally restricted	10c , 10d	Strong expression in the circular smooth muscle layer, and weaker in or absent from various anterior-posterior levels of the longitudinal smooth muscle layer.
TS25: enteric nervous system	Absent		10d , 10c
TS25: intestine epithelium	Absent		10d , 10c

Structure	Level	Pattern	Image	Note
TS23: blood vessel smooth muscle	Present		11a , 11b

Structure	Level	Pattern	Image	Note
TS25: blood vessel	Present	Regionally restricted	11c , 11d inset , 11c inset , 11d

Structure	Level	Pattern	Image	Note
TS20: esophagus	Absent

Structure	Level	Pattern	Image	Note
TS21: esophagus	Absent

Structure	Level	Pattern	Image	Note
TS28: cecum	Present	Regionally restricted		Expressed in the circular muscle layer.
TS28: large intestine smooth muscle	Present	Regionally restricted		Expressed in the circular muscle layer.

Structure	Level	Pattern	Image	Note
TS21: intestine mesenchyme	Present	Diffuse	S1b , S1a	Expressed throughout the large intestine mesenchyme.
TS21: enteric nervous system	Absent		S1a , S1b
TS21: intestine epithelium	Absent		S1a , S1b

Structure	Level	Pattern	Image	Note
TS21: intestine mesenchyme	Present	Regionally restricted	S1d , S1c	Expressed in both layers of the forming muscle wall in the large intestine.
TS21: enteric nervous system	Absent		S1d , S1c
TS21: intestine epithelium	Absent		S1d , S1c

Structure	Level	Pattern	Image	Note
TS21: intestine mesenchyme	Present	Regionally restricted	S1e , S1f	Expressed in both layers of the forming muscle wall in the large intestine.
TS21: enteric nervous system	Absent		S1e , S1f
TS21: intestine epithelium	Absent		S1e , S1f

Structure	Level	Pattern	Image	Note
TS23: intestine muscularis	Strong	Regionally restricted	S2b , S2a	Strong expression in the circular smooth muscle layer, but absent from the longitudinal smooth muscle layer in the large intestine.
TS23: enteric nervous system	Absent		S2a , S2b
TS23: intestine epithelium	Absent		S2a , S2b

Structure	Level	Pattern	Image	Note
TS23: intestine muscularis	Strong	Regionally restricted	S2c	Strong expression in the circular smooth muscle layer and the longitudinal smooth muscle layer in the large intestine.
TS23: enteric nervous system	Absent		S2c
TS23: intestine epithelium	Absent		S2c

Structure	Level	Pattern	Image	Note
TS23: intestine muscularis	Strong	Regionally restricted	S2d	Strong expression in the circular smooth muscle layer, and weaker expression in the longitudinal smooth muscle layer in the large intestine.
TS23: enteric nervous system	Absent		S2d
TS23: intestine epithelium	Absent		S2d

Structure	Level	Pattern	Image	Note
TS28: large intestine smooth muscle	Present	Regionally restricted	S3b , S3a	Expressed in the circular muscle layer.
TS28: intrinsic nerve plexus	Absent		S3a , S3b
TS28: large intestine mucosa	Absent		S3a , S3b

Structure	Level	Pattern	Image	Note
TS28: large intestine smooth muscle	Present	Regionally restricted	S3c , S3d	Expressed in the circular muscle layer.
TS28: intrinsic nerve plexus	Absent		S3c , S3d
TS28: large intestine mucosa	Absent		S3c , S3d