Antibody:	Integrin beta2 Antibody (K-19)
Detection system:	Secondary antibody coupled to other - see notes
Assay notes:	Secondary antibody was labelled either with FITC or Cy3.

Structure	Level	Pattern	Image	Note
TS17: endocardial lining	Present		2A
TS17: cardiac jelly	Present	Regionally restricted	2A	Expressed on the syrface of mesenchymal cells in the cardiac jelly.
TS17: heart mesenchyme	Present	Regionally restricted	2A	Expressed on the syrface of mesenchymal cells in the cardiac jelly.
TS17: visceral pericardium	Present		2A	Expressed in epicardium.
TS17: cardiac muscle tissue	Weak	Regionally restricted	2A	Expressed in some areas of the myocardium.

Structure	Level	Pattern	Image	Note
TS21: endocardial tissue	Strong	Regionally restricted	2C	Expressed in the endothelium cells.
TS21: heart mesenchyme	Strong	Regionally restricted	2C	Expressed in mesenchymal cells in the cardiac jelly.

Structure	Level	Pattern	Image	Note
TS23: endocardial lining	Present	Regionally restricted	2D	Expressed in the valve endothelial cells.
TS23: cardiac valve leaflet	Present	Regionally restricted	2D	Expressed in fibroblast-like interstitial cells in valve leaflets.

Structure	Level	Pattern	Image	Note
TS25: endocardial tissue	Present	Regionally restricted	2E	Expressed in the valve endothelial cells.
TS25: cardiac valve leaflet	Present	Regionally restricted	2E	Expressed in fibroblast-like interstitial cells in valve leaflets.

Structure	Level	Pattern	Image	Note
TS26: endocardial tissue	Present	Regionally restricted	2F	Weaker expression in the valve endothelial cells.
TS26: cardiac valve leaflet	Present	Regionally restricted	2F	Weaker expression in fibroblast-like interstitial cells in valve leaflets.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present	Regionally restricted	2G	Expressed in both the mural and trabecular portions of the myocardium.
TS23: visceral pericardium	Strong		2G	Expressed in epicardium.

Structure	Level	Pattern	Image	Note
TS28: thymus	Strong		2H