Gene Expression Data
Assay Details
Assay
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Reference:
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J:72548
Brachtendorf G, et al., Early expression of endomucin on endothelium of the mouse embryo and on putative hematopoietic clusters in the dorsal aorta. Dev Dyn. 2001 Nov;222(3):410-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5637528
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Gene symbol:
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Emcn
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Gene name:
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endomucin
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Antibody:
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anti-Endomucin (V.5C7; sc-53941)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The authors developed three monoclonal antibodies but did not specify which was used to generate the data in Figs 3 and 5; it is possible V.1A7 (MGI:5637518) or even V.7C7 (MGI:5637519) was used to generate those data.
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|
Results
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Specimen
3A embryo:
embryonic day 7.0
(more )
Note: transverse section
Specimen
3A embryo:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 7.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Note:
|
transverse section
|
Specimen
3A mother:
postnatal adult
(more )
Note: transverse section
Specimen
3A mother:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
transverse section
|
Specimen
3D:
embryonic day 8.0
(more )
Note: parasagittal section
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
parasagittal section
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: blood vessel |
Strong |
|
3D
|
Expression is present in intersomitic vessels.
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Specimen
3G/H/I:
embryonic day 8.0
(more )
Note: transverse section
Specimen
3G/H/I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
transverse section
|
Specimen
3G mother:
postnatal adult
(more )
Note: transverse section
Specimen
3G mother:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
transverse section
|
Specimen
3K:
embryonic day 8.5-9.0
(more )
Note: parasagittal section
Specimen
3K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5-9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
parasagittal section
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: blood vessel |
Present |
|
3K
|
Expression is present in the endothelium of capillaries and large blood vessels throughout the embryo.
|
Specimen
4A/B/C:
embryonic day 11.5
(more )
Specimen
4A/B/C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dorsal aorta |
Strong |
Clusters |
4C
,
4A
,
4B
|
Expression is present on the endothelium of the aorta, as well as on cell clusters associated with the endothelium; they may be hmatopoietic progenitors.
|
Specimen
4L:
embryonic day 15.5
(more )
Specimen
4L:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: blood vessel |
Present |
|
4L
|
Expression is present in venules.
|
TS23: blood |
Present |
|
4L
|
The stained cell could be either a circulating endothelial precursor or a hematopoietic progenitor.
|
Specimen
5A/D:
embryonic day 15.5
(more )
Specimen
5A/D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5G:
postnatal adult
(more )
Note: transverse section
Specimen
5G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
transverse section
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: aorta |
Present |
|
5G
|
Focal expression is observed.
|
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