Gene Expression Data
Assay Details
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Assay
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Reference:
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J:150047
Lei K, et al., SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice. Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10207-12
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5637338
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Gene symbol:
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Syne1
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Gene name:
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spectrin repeat containing, nuclear envelope 1
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Antibody:
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anti-Syne-1
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
1A:
embryonic day 18.5
(more  )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
1A:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: transversus thoracis |
Present |
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1A
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1B
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1C
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Expression is localized to the nuclear envelope of both synaptic and nonsynaptic nuclei.
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Specimen
1D:
embryonic day 18.5; Sun1tm1Mhan/ Sun1tm1Mhan
(more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
1D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1tm1Mhan
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: transversus thoracis |
Present |
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1D
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Expression is localized to the nuclear envelope of synaptic nuclei; the number of these nuclei is reduced in the mutant compared to wild type.
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Specimen
1E:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1tm1Mhan
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: transversus thoracis |
Present |
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1E
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Expression is localized to the nuclear envelope of nonsynaptic nuclei; the distribution of these nuclei in these mice appears similar to wild type.
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Specimen
2C WT:
postnatal adult
(more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
2C WT:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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Specimen
2C +/-; +/-:
postnatal adult; Sun1tm1Mhan/Sun1 +, Sun2tm1Mhan/Sun2 +
(more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
2C +/-; +/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1+
Sun2tm1Mhan/Sun2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibialis anterior |
Present |
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2C upper right
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Expression is localized to the nuclear envelope of synaptic nuclei; the distribution of these nuclei in these mice appears similar to wild type.
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Specimen
2C -/-; +/-:
postnatal adult; Sun1tm1Mhan/ Sun1tm1Mhan, Sun2tm1Mhan/Sun2 +
(more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
2C -/-; +/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1tm1Mhan
Sun2tm1Mhan/Sun2+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibialis anterior |
Present |
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2C lower left
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Expression is localized to the nuclear envelope of synaptic nuclei; the number of these nuclei is reduced in the mutant compared to wild type.
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Specimen
2C +/-; -/-:
postnatal adult; Sun1tm1Mhan/Sun1 +, Sun2tm1Mhan/ Sun2tm1Mhan
(more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
2C +/-; -/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1+
Sun2tm1Mhan/Sun2tm1Mhan
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibialis anterior |
Present |
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2C lower right
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Expression is localized to the nuclear envelope of synaptic nuclei; the number of these nuclei is reduced in the mutant compared to wild type.
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Specimen
5A:
embryonic day 18.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1+
Sun2tm1Mhan/Sun2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: intercostal muscle |
Present |
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5D
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5F
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Expression is localized in the nuclear envelope; no differences in localization between these mice and wild type were observed.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1tm1Mhan
Sun2tm1Mhan/Sun2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: intercostal muscle |
Present |
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5I
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5G
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Expression is localized in the nuclear envelope; however, the amount of localization is reduced compared to that seen in wild type mice.
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Specimen
5J:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Sun1tm1Mhan/Sun1+
Sun2tm1Mhan/Sun2tm1Mhan
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: intercostal muscle |
Present |
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5L
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5J
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Expression is localized in the nuclear envelope; however, the amount of localization is reduced compared to that seen in wild type mice.
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Specimen
5M:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: intercostal muscle |
Ambiguous |
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5M
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5O
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No localization to the nuclear envelope is seen in the double mutant.
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Specimen
S1F:
postnatal adult
(more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Specimen
S1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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Note:
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The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: skeletal muscle |
Present |
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S1F
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Expression is present in nuclei at the neuromuscular junction.
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