Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:68905
Simonian SX, et al., Regulation of gonadotropin-releasing hormone (GnRH) gene expression during GnRH neuron migration in the mouse. Neuroendocrinology. 2001 Mar;73(3):149-56
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5636503
|
Gene symbol:
|
Gnrh1
|
Gene name:
|
gonadotropin releasing hormone 1
|
Probe:
|
probe 2
|
Probe preparation:
|
Antisense labelled with S35 DNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
1A:
embryonic day 14.5
(more )
Note: Sections were fixed with 4% paraformaldehyde.
Specimen
1A:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA/Ca
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Sections were fixed with 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forebrain |
Present |
Regionally restricted |
1A
|
Expression was detected in the medial septum and preoptic area of the basal forebrain.
|
Specimen
1B:
embryonic day 14.5
(more )
Note: Sections were fixed with 4% paraformaldehyde.
Specimen
1B:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA/Ca
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Sections were fixed with 4% paraformaldehyde.
|
Specimen
not shown E12.5:
embryonic day 12.5
(more )
Note: Sections were fixed with 4% paraformaldehyde.
Specimen
not shown E12.5:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA/Ca
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Sections were fixed with 4% paraformaldehyde.
|
Specimen
not shown E16.5:
embryonic day 16.5
(more )
Note: Sections were fixed with 4% paraformaldehyde.
Specimen
not shown E16.5:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA/Ca
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Sections were fixed with 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: nasal septum |
Present |
|
|
|
TS24: forebrain |
Present |
Regionally restricted |
|
Expression was detected in the medial septum and preoptic area of the basal forebrain.
|
Specimen
not shown E19.5:
embryonic day 19.5
(more )
Note: Sections were fixed with 4% paraformaldehyde.
Specimen
not shown E19.5:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA/Ca
|
Age:
|
embryonic day 19.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Note:
|
Sections were fixed with 4% paraformaldehyde.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: nasal septum |
Present |
|
|
There was a significant increase in silver grain density at E19.5 compared to E12.5.
|
TS26: forebrain |
Present |
Regionally restricted |
|
Expression was detected in the medial septum and preoptic area of the basal forebrain. There was a significant increase in silver grain density at E19.5 compared to E14.5 and E16.5.
|
|