Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46409
Hirsch MR, et al., Control of noradrenergic differentiation and Phox2a expression by MASH1 in the central and peripheral nervous system. Development. 1998 Feb;125(4):599-608
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5636469
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Gene symbol:
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Dbh
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Gene name:
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dopamine beta hydroxylase
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Probe:
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DBH probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1I:
embryonic day 10.5; Ascl1tm1And/Ascl1 +
(more  )
Specimen
1I:
(close  )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
1J:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inferior cervical ganglion |
Present |
Regionally restricted |
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The few surviving sympathoblasts are labelled in the rudimentary ganglion in the mutant.
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Specimen
2E:
embryonic day 12.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
2E:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: esophagus |
Present |
Regionally restricted |
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Expression was detected in the esphageal wall.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
5C:
postnatal newborn; Ascl1tm1And/Ascl1 +
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Specimen
5D:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Specimen
5K:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
5K:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pons |
Present |
Regionally restricted |
5K
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Expression was detected in the locus coeruleus anlage.
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| TS21: brainstem |
Absent |
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5K
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Expression was absent in the reticular formation.
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Specimen
5L:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: brainstem |
Absent |
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5L
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Expression was absent in the reticular formation.
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| TS21: pons |
Absent |
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5L
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There was no expression in the expected location of the locus coeruleus neurons, which appear to never develop in the mutant.
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Specimen
5M:
embryonic day 13.5; Ascl1tm1And/Ascl1 +
(more )
Specimen
5M:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindbrain |
Present |
Regionally restricted |
5M
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Expression was detected in (nor)adrenergic cells in areas A1 and C1.
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Specimen
5N:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hindbrain |
Absent |
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5N
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Expression was absent in areas A1 and C1.
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Specimen
6A:
embryonic day 10.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
6A
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Expression was detected in the locus coeruleus precursors.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Ambiguous |
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6B
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There was no expression in the expected location of the locus coeruleus neurons, which appear to never develop in the mutant.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129 * CD-1
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Analysis Tools