About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:46409 Hirsch MR, et al., Control of noradrenergic differentiation and Phox2a expression by MASH1 in the central and peripheral nervous system. Development. 1998 Feb;125(4):599-608
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5636467
Gene symbol: Phox2a
Gene name: paired-like homeobox 2a
Antibody: anti-Phox2a
Results
Specimen 1A: embryonic day 10.5; Ascl1tm1And/Ascl1+ (more )
Note: Section through the cervical region. Double labeled: orange - Phox2a; black - Ret (assay MGI:5636466).
Structure Level Pattern Image Note
TS17: sympathetic nervous system Present Regionally restricted 1A Expression was detected in sympathetic neuroblasts.
Specimen 1B: embryonic day 10.5; Ascl1tm1And/Ascl1tm1And (more )
Note: Section through the cervical region. Double labeled: orange - Phox2a; black - Ret (assay MGI:5636466).
Structure Level Pattern Image Note
TS17: sympathetic nervous system Absent 1B Mutant sympathetic neuroblasts failed to express this protein.
Specimen 1E: embryonic day 10.5; Ascl1tm1And/Ascl1+ (more )
Note: Section through the thoracic region.
Structure Level Pattern Image Note
TS17: sympathetic nervous system Present Regionally restricted 1E Expression was detected in sympathetic neuroblasts.
Specimen 1F: embryonic day 10.5; Ascl1tm1And/Ascl1tm1And (more )
Note: Section through the thoracic region.
Structure Level Pattern Image Note
TS17: sympathetic nervous system Absent 1F Mutant sympathetic neuroblasts failed to express this protein.
Specimen 1M: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: inferior cervical ganglion Present Regionally restricted 1M Expression was detected in sympathetic neuroblasts.
Specimen 1N: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: inferior cervical ganglion Present Regionally restricted 1N The few surviving sympathoblasts are labelled in the rudimentary ganglion in the mutant.
Specimen 2C: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: parasympathetic ganglion Present 2C
TS21: sphenopalatine ganglion Present 2C
Specimen 2D: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: sphenopalatine ganglion Absent 2D
Specimen 2I: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: otic ganglion Present 2I
TS21: parasympathetic ganglion Present 2I
TS21: facial ganglion Present 2I Expression was detected in the geniculate ganglion.
Specimen 2J: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: otic ganglion Absent 2J
TS21: parasympathetic ganglion Absent 2J
TS21: facial ganglion Present 2J Expression was detected in the geniculate ganglion, as in wild type.
Specimen 3C: embryonic day 16.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS24: sphenopalatine ganglion Present 3C
Specimen 3D: embryonic day 16.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS24: sphenopalatine ganglion Weak 3D The few remaining cells in the mutant atrophic sphenopalatine ganglion show weak expression.
Specimen 4C: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: esophagus Present Regionally restricted 4C Expression was detected in the myenteric plexus of the stomach and lower esophagus.
TS21: stomach Present Regionally restricted 4C Expression was detected in the myenteric plexus of the stomach and lower esophagus.
Specimen 4D: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: stomach Present Regionally restricted 4D Expression was detected in the myenteric plexus of the stomach, as in wild type.
TS21: esophagus Absent 4D The presumptive myenteric neurons of the lower esophagus were negative.
Specimen 4G: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: esophagus Present Regionally restricted 4G Expression was detected in the myenteric plexus of the upper esophagus.
Specimen 4H: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: esophagus Absent 4H There was no staining in the myenteric plexus of the upper esophagus.
Specimen 5E: postnatal newborn; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS27: locus coeruleus Present 5E
TS27: pontine reticular formation Present 5E
Specimen 5F: postnatal newborn; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS27: pontine reticular formation Present 5F
Specimen 5G: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: pons Present Regionally restricted 5G Expression was detected in the locus coeruleus anlage.
TS21: brainstem Present Regionally restricted 5G Expression was detected in the reticular formation.
Specimen 5H: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: brainstem Present Regionally restricted 5H Expression was detected in the reticular formation.
TS21: pons Ambiguous 5H There was no expression in the expected location of the locus coeruleus neurons, which appear to never develop in the mutant.
Specimen 7I: embryonic day 13.5; Ascl1tm1And/Ascl1+ (more )
Structure Level Pattern Image Note
TS21: alar columns Present Regionally restricted 7I Expression was found in a discrete column of cells in the lateral neural tube just dorsal to the sulcus limitans.
Specimen 7J: embryonic day 13.5; Ascl1tm1And/Ascl1tm1And (more )
Structure Level Pattern Image Note
TS21: alar columns Absent 7J
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory