Gene Expression Data
Assay Details
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Assay
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Reference:
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J:61910
Kramer PR, et al., Transcription factor activator protein-2 is required for continued luteinizing hormone-releasing hormone expression in the forebrain of developing mice. Endocrinology. 2000 May;141(5):1823-38
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5636450
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Gene symbol:
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Gnrh1
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Gene name:
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gonadotropin releasing hormone 1
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Antibody:
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anti-pro-LHRH antibody (SW-1)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The authors described two antibodies but did not specify which was used to generate these data; it is possible MGI:5467573 was used instead.
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Results
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Specimen
1E/G:
embryonic day 14.5
(more  )
Note: Overlay of adjacent sections; brown - Gnrh1; silver grains - Tfap2a (assay MGI:5636453).
Specimen
1E/G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Overlay of adjacent sections; brown - Gnrh1; silver grains - Tfap2a (assay MGI:5636453).
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Specimen
6A:
embryonic day 13.5
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6B:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forebrain |
Present |
Single cells |
6B
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The number of expressing cells is similar in mutant and wild type.
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Specimen
6C:
embryonic day 14.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6D:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: forebrain |
Present |
Single cells |
6D
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The number of expressing cells is significantly reduced in the mutant compared to wild type.
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Specimen
not shown WT:
embryonic day 15.5
(more )
Specimen
not shown WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown mutant:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forebrain |
Present |
Single cells |
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The number of expressing cells is significantly reduced in the mutant compared to wild type.
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Specimen
8G:
embryonic day 14.5
(more )
Specimen
8G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tectum |
Present |
Single cells |
8G
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Specimen
8H:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: tectum |
Present |
Single cells |
8H
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Expression is as in wild type.
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Specimen
8I:
embryonic day 15.5
(more )
Specimen
8I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tectum |
Present |
Single cells |
8I
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Specimen
8J:
(close )
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Genetic Background:
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either: (involves: 129S4/SvJae) or (involves: 129S4/SvJae * BALB/c)
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Mutant Allele(s):
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Tfap2atm1Jae/Tfap2atm1Jae
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tectum |
Present |
Single cells |
8J
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Expression is as in wild type.
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Analysis Tools