Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:178869 Yun EJ, et al., mSmile is Necessary for Bronchial Smooth Muscle and Alveolar Myofibroblast Development. Anat Rec (Hoboken). 2012 Jan;295(1):167-76
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5635683
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Results

Specimen 4A: postnatal day 3 (more

)

Structure	Level	Pattern	Image	Note
TS27: alveolar septum	Present		4A
TS27: lung	Present	Regionally restricted	4A	Expressed in cells around proximal airways and blood vessels.

Specimen 4B: postnatal day 3; Tmtc3^{Gt(JST185)Byg}/Tmtc3^{Gt(JST185)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS27: lung	Present	Regionally restricted	4B	Expressed in cells around proximal airways and blood vessels.
TS27: alveolar septum	Absent		4B

Specimen 4C: postnatal day 5 (more

)

Structure	Level	Pattern	Image	Note
TS28: lung parenchyma	Present	Widespread	4C

Specimen 4D: postnatal day 5; Tmtc3^{Gt(JST185)Byg}/Tmtc3^{Gt(JST185)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS28: lung parenchyma	Present		4D	Not as numerous as in the wild-type.

Specimen 4G: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: bronchus mesenchyme	Present	Regionally restricted	4G	Expressed in cells surrounding proximal bronchioles and at the clefts of branching terminal epithelial buds.

Specimen 4H: embryonic day 14.5; Tmtc3^{Gt(JST185)Byg}/Tmtc3^{Gt(JST185)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS22: bronchus mesenchyme	Present	Regionally restricted	4H	Expression was reduced surrounding the proximal airways and was rarely found at the clefts.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24