Gjb4 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:32852 Davies TC, et al., Multiple members of the connexin gene family participate in preimplantation development of the mouse. Dev Genet. 1996;18(3):234-43
Assay type:	RT-PCR
MGI Accession ID:	MGI:5635575
Gene symbol:	Gjb4
Gene name:	gap junction protein, beta 4

Probe:	Gjb4-pC, Gjb4-pD
Assay notes:	Semi-quantitative RT-PCR was used to obtain these results; alpha-globin was used as the standard.

Results

Image: 3_Cx30.3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
16h post hCG	P (a)	TS28: secondary oocyte	Ambiguous	Not Specified; total RNA	CF-1		Female	ovulated oocyte
48h post hCG	E1.0 (a)	TS2: 2-cell stage conceptus	Ambiguous	Not Specified; total RNA	involves: BALB/c * C57BL/6J * CF-1		Not Specified
60h post hCG	E2.0 (a)	TS3: 4-cell stage conceptus	Present	Not Specified; total RNA	involves: BALB/c * C57BL/6J * CF-1		Not Specified
65h post hCG	E2.5 (a)	TS3: 8-cell stage conceptus	Present	Not Specified; total RNA	involves: BALB/c * C57BL/6J * CF-1		Not Specified
74h post hCG	E2.5 (a)	TS3: morula-stage conceptus	Present	Not Specified; total RNA	involves: BALB/c * C57BL/6J * CF-1		Not Specified
82h post hCG	E3.0 (a)	TS4: morula-stage conceptus	Present	Not Specified; total RNA	involves: BALB/c * C57BL/6J * CF-1		Not Specified
92h post hCG	E3.5 (a)	TS4: blastocyst-stage conceptus	Present	Not Specified; total RNA	involves: BALB/c * C57BL/6J * CF-1		Not Specified

Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Transcripts accumulate beginning in the eight-cell stage. Transcripts could be detected before the eight-cell stage if larger numbers of embryos and more amplification cycles were used.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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