Gene Expression Data
Assay Details
Assay
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Reference:
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J:198561
Mikedis MM, et al., Widespread but tissue-specific patterns of interferon-induced transmembrane protein 3 (IFITM3, FRAGILIS, MIL-1) in the mouse gastrula. Gene Expr Patterns. 2013 Oct;13(7):225-39
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5635467
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Gene symbol:
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Runx1
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Gene name:
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runt related transcription factor 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
5F:
embryonic day 8.25; Runx1tm2Spe/Runx1 +
(more )
Note: 4 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
Specimen
5F:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA/J
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 8.25
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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4 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
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Structure |
Level |
Pattern |
Image |
Note |
TS12: allantois |
Present |
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5F
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TS12: primitive streak |
Present |
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5F
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TS12: embryo |
Present |
Regionally restricted |
5F
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Expression is in the posterior dorsal epiblast.
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TS12: amnion ectoderm |
Present |
Regionally restricted |
5F
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Expression is in the amniotic ectoderm adjacent to the posterior region, allantois- and primitive streak-associated posterior visceral endoderm/hindgut.
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Specimen
5G:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
Specimen
5G:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA/J
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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6 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
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Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo endoderm |
Present |
Regionally restricted |
5G
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Expression is in the anterior endoderm overlying the heart, yolk sac hematopoietic cells.
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Specimen
5H:
embryonic day 9.0; Runx1tm2Spe/Runx1 +
(more )
Note: 9 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
Specimen
5H:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA/J
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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9 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
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Specimen
5I1:
embryonic day 9.0; Runx1tm2Spe/Runx1 +
(more )
Note: 12 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
Specimen
5I1:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA/J
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
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Structure |
Level |
Pattern |
Image |
Note |
TS14: tail bud |
Present |
Regionally restricted |
5I1
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Expression is in the ventral ectodermal ridge.
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Specimen
5I2:
embryonic day 9.0; Runx1tm2Spe/Runx1 +
(more )
Note: 12 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
Specimen
5I2:
(close )
Genetic Background:
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involves: 129S4/SvJae * C57BL/6J * CBA/J
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Mutant Allele(s):
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Runx1tm2Spe/Runx1+
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12 somites. Double labeled: blue - Runx1 reporter; brown - Ifitm3 (assay MGI:5635440).
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