Antibody:

Anti-Prox1

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle	Present	Regionally restricted	4D , 4A	Expression was detected in cardiac myocytes.

Structure	Level	Pattern	Image	Note
TS21: lymphatic system	Present	Regionally restricted	5A	Expression was detected in lymphatic vessels found between the aorta and pulmonary trunk. There was no co-localization with Lyve1.

Structure	Level	Pattern	Image	Note
TS23: lymphatic system	Present	Regionally restricted	7A	Expression was on lymphatic vessels that had begun to extend from the great vessels toward the ventricles.

Structure	Level	Pattern	Image	Note
TS23: lymphatic system	Present	Regionally restricted	7C	Expression was detected in the lymphatics encircling the great vessels. There was co-localization with Lyve1 staining.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Absent		7E	Lyve1-positive cells were Prox1 negative.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	8A	Expression was in lymphatic vessels at the atrioventricular junction.

Structure	Level	Pattern	Image	Note
TS28: lymphatic vessel	Present	Regionally restricted	8E	Expression was in epicardial lymphatic vessels covering the ventricles.
TS28: visceral pericardium	Present	Regionally restricted	8E	Expression was in epicardial lymphatic vessels covering the ventricles.

Structure	Level	Pattern	Image	Note
TS28: ventricle cardiac muscle	Absent		8I