Lhx9 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212053 Takasawa K, et al., FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice. FASEB J. 2014 May;28(5):2020-8
Assay type:	RT-PCR
MGI Accession ID:	MGI:5635214
Gene symbol:	Lhx9
Gene name:	LIM homeobox protein 9

Probe:	Lhx9-pE, Lhx9-pF
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression is shown as a graph normalized to Rps29 expression.

Results

Image: 1A Lhx9

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
11.5	E11.5	TS19: gonad	Present (a)	0.2 µg; total RNA	ICR		Male
11.5	E11.5	TS19: gonad	Present (a)	0.2 µg; total RNA	ICR		Female
12.5	E12.5	TS20: testis	Present (a)	0.2 µg; total RNA	ICR		Male
12.5	E12.5	TS20: ovary	Present (a)	0.2 µg; total RNA	ICR		Female
13.5	E13.5	TS21: testis	Present (a)	0.2 µg; total RNA	ICR		Male
13.5	E13.5	TS21: ovary	Present (a)	0.2 µg; total RNA	ICR		Female
14.5	E14.5	TS22: testis	Present (b)	0.2 µg; total RNA	ICR		Male
14.5	E14.4	TS22: ovary	Present (b)	0.2 µg; total RNA	ICR		Female

Notes:
(a) Expression is similar between male and female gonads.
(b) Expression is similar between male and female gonads. Expression levels did not change dramatically between 11.5-14.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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