Antibody:	anti-LTBP Ab39
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS12: embryo	Absent			Authors report no expression at the early stage of 8.5 ED, before the onset of neural groove closure, in ectoderm, mesoderm or endoderm derivatives.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	1A	Expression in extracellular matrix distributed on basal surface of neural tube and somite.
TS13: somite	Present	Regionally restricted	1A	Expression in extracellular matrix distributed on basal surface of neural tube and somite.
TS13: migrating neural crest	Present	Regionally restricted	1A	Extracellular fibrillar arrangement of expression surrounding migrating neural crest cells.
TS13: embryo ectoderm	Absent		1A	No expression on basal surface of ectoderm or coelomic epithelium.
TS13: intraembryonic coelom	Absent		1A	No expression on basal surface of ectoderm or coelomic epithelium.

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Absent

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present	Regionally restricted	1B	Extracellular fibrillar arrangement of expression surrounding migrating neural crest cells.

Structure	Level	Pattern	Image	Note
TS13: outflow tract endocardial tube	Present	Regionally restricted	2A1	Expression on basal surface of endocardium and myocardium of outflow tract.
TS13: outflow tract mesenchyme	Present	Regionally restricted	2A1	Expression on basal surface of endocardium and myocardium of outflow tract.
TS13: common atrial chamber cardiac muscle	Absent		2A1	No expression on basal surface of endocardium or myocardium of atrioventricular canal.
TS13: common atrial chamber endocardial tube	Absent		2A1	No expression on basal surface of endocardium or myocardium of atrioventricular canal.
TS13: primitive ventricle cardiac muscle	Absent		2A1	No expression on basal surface of endocardium or myocardium of atrioventricular canal.
TS13: primitive ventricle endocardial tube	Absent		2A1	No expression on basal surface of endocardium or myocardium of atrioventricular canal.
TS13: cardiac jelly	Absent		2A1	No expression within extracellular matrix of acellular cardiac jelly.

Structure	Level	Pattern	Image	Note
TS15: heart mesenchyme	Present	Regionally restricted	2B1	Extracellular fibrillar expression surrounding migrating mesenchymal cells in atrioventricular region.

Structure	Level	Pattern	Image	Note
TS15: outflow tract mesenchyme	Present	Regionally restricted	2B2	Extracellular fibrillar expression surrounding migrating mesenchymal cells of outflow tract.

Structure	Level	Pattern	Image	Note
TS19: endocardial cushion	Present	Regionally restricted	2C1	Extracellular fibrillar expression surrounding mesenchymal cells within cushion tissue of atrioventricular canal.

Structure	Level	Pattern	Image	Note
TS19: outflow tract cardiac muscle	Present	Regionally restricted	2C2	Expression distributed on basal surface of myocardium of outflow tract.
TS19: outflow tract	Present	Regionally restricted	2C2	No expression in mesenchymal tissue of outflow tract.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	3A	Extracellular fibrillar expression on basal surface of endothelium and in mesenchymal cells of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present	Regionally restricted	3B1	Extracellular expression on basal surface of endothelium and surface of smooth muscle progenitor cells of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present	Regionally restricted	3C1	Expression on basal surface of endothelium and surface of smooth muscle progenitor cells of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS17: visceral pleura	Present	Regionally restricted	4A1	Expression on basal surface of coelomic epithelia (visceral pleura originating from lateral plate mesoderm).
TS17: lung epithelium	Present	Regionally restricted	4A1	Expression on basal surface of cuboidal epithelium of developing bronchus of lung bud (originating from endoderm of foregut).
TS17: lung mesenchyme	Present	Regionally restricted	4A1	Extracellular fibrillar expression in mesenchymal tissue between epithelia.

Structure	Level	Pattern	Image	Note
TS17: lung	Present	Regionally restricted		No expression at basal surface of terminal end of developing lung bud.

Structure	Level	Pattern	Image	Note
TS21: trachea mesenchyme	Present	Regionally restricted		Extracellular fibrillar expression in mesenchyme in ventral region of tracheal wall (primordium of tracheal cartilage).

Structure	Level	Pattern	Image	Note
TS17: peritoneum visceral mesothelium	Present	Regionally restricted	4B1	Expression on basal surface of visceral peritoneum of the liver.
TS17: liver	Absent		4B1	No expression in liver cords.

Structure	Level	Pattern	Image	Note
TS17: peritoneum visceral mesothelium	Present	Regionally restricted	4B2	Fibrillar network of expression in visceral peritoneum of the liver.

Structure	Level	Pattern	Image	Note
TS19: peritoneum visceral mesothelium	Present	Regionally restricted	4C1	Expression on basal surface of visceral peritoneum of intestine.
TS19: intestine epithelium	Present	Regionally restricted	4C1	Expression on basal surface of intestinal epithelium.
TS19: intestine mesenchyme	Present	Regionally restricted	4C1	Extracellular fibrillar expression in mesenchymal tissue between epithelia.

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present	Regionally restricted	5A1	Extracellular fibrillar expression within mesenchymal condensation that will later form the humerus.

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present	Regionally restricted	5A2	Extracellular expression within mesenchymal condensation that will later form the humerus.

Structure	Level	Pattern	Image	Note
TS21: humerus cartilage condensation	Present	Regionally restricted	5B	No expression in chondrocytes or perichondrium.

Structure	Level	Pattern	Image	Note
TS19: head	Present	Regionally restricted	5C	Extracellular expression within mesenchymal tissue of temporal bone.