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Gene Expression Data
Assay Details
Assay
Reference: J:166815 Yoshikawa F, et al., Phospholipase d family member 4, a transmembrane glycoprotein with no phospholipase D activity, expression in spleen and early postnatal microglia. PLoS One. 2010;5(11):e13932
Assay type: RNA in situ
MGI Accession ID: MGI:5633980
Gene symbol: Pld4
Gene name: phospholipase D family member 4
Probe: Pld4 cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Fluorescein
Assay notes: Probe is alkaline-hydrolyzed, and HNPP/Fast Red fluorescent detection was used.
Results
Specimen 5C upper Cerebral cortex: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Aif1 (assay MGI:5633995).
Structure Level Pattern Image Note
TS28: cortical layer II Present Scattered 5C upper Cerebral cortex Co-expressed with Aif1 in microglial cells.
TS28: cortical layer III Present Scattered 5C upper Cerebral cortex Co-expressed with Aif1 in microglial cells.
Specimen 5C upper Hippocampal: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Aif1 (assay MGI:5633995).
Structure Level Pattern Image Note
TS28: hippocampus CA1 Present Scattered 5C upper Hippocampal Co-expressed with Aif1 in microglial cells. Expressed in the stratumlacunosum-moleculare.
Specimen 5C upper Inferior colliculus: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Aif1 (assay MGI:5633995).
Structure Level Pattern Image Note
TS28: inferior colliculus Present Scattered 5C upper Inferior colliculus Co-expressed with Aif1 in microglial cells.
Specimen 5C upper Cerebellar: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Aif1 (assay MGI:5633995).
Structure Level Pattern Image Note
TS28: cerebellum white matter Present Scattered 5C upper Cerebellar Co-expressed with Aif1 in microglial cells.
Specimen 5C lower Cerebral cortex: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Gfap (assay MGI:5633993).
Structure Level Pattern Image Note
TS28: cortical layer II Present Scattered 5C lower Cerebral cortex Do not co-express GFAP.
TS28: cortical layer III Present Scattered 5C lower Cerebral cortex Do not co-express GFAP.
Specimen 5C lower Hippocampal: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Gfap (assay MGI:5633993).
Structure Level Pattern Image Note
TS28: hippocampus CA1 Present Scattered 5C lower Hippocampal Do not co-express GFAP. Expressed in the stratumlacunosum-moleculare.
Specimen 5C lower Inferior colliculus: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Gfap (assay MGI:5633993).
Structure Level Pattern Image Note
TS28: inferior colliculus Present Scattered 5C lower Inferior colliculus Do not co-express GFAP.
Specimen 5C lower Cerebellar: postnatal day 7 (more )
Note: Double labeled: red - Pld4; green - Gfap (assay MGI:5633993).
Structure Level Pattern Image Note
TS28: cerebellum white matter Present Scattered 5C lower Cerebellar Do not co-express GFAP.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory