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Gene Expression Data
Assay Details
Assay
Reference: J:204143 Wang L, et al., The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming. Cell Stem Cell. 2013 Dec 5;13(6):676-90
Assay type: RT-PCR
MGI Accession ID: MGI:5632375
Gene symbol: Thoc5
Gene name: THO complex 5
Probe: Thoc5-pA, Thoc5-pB
Probe preparation: labelled with EvaGreen
Assay notes: Quantitative RT-PCR. Relative expression was normalized to the one-cell stage and Actb was used as the standard.
Results Image: 6E Thoc5
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
1-cell E0.5 (a) TS1: 1-cell stage conceptus Present 0.5 µg; total RNA Not Specified Not Specified
2-cell E1.0 (a) TS2: 2-cell stage conceptus Present 0.5 µg; total RNA Not Specified Not Specified
4/8 cell E2.0-2.5 (a) TS3: 4-8 cell stage conceptus Present 0.5 µg; total RNA Not Specified Not Specified
Morula E3.0 (a) TS3: morula-stage conceptus Present 0.5 µg; total RNA Not Specified Not Specified
Blastocyst E3.5 (a) TS4: blastocyst-stage conceptus Present (b) 0.5 µg; total RNA Not Specified Not Specified
- Other lane(s) in blot assay different gene(s)
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression increased 11.1 fold, compared to one-cell embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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