Antibody:	anti-EGFR
Detection system:	Secondary antibody coupled to biotin
Assay notes:	Fixative was either Carnoy's or Bouin's solution. A Biotinyltyramide-based Catalyzed Signal Amplification Method was used to visualize signal.

Structure	Level	Pattern	Image	Note
TS21: submandibular gland primordium epithelium	Present		4A	Expression is in cell membranes.
TS21: submandibular gland primordium mesenchyme	Ambiguous		4A	The authors state that staining on mesenchymal cells was equivocal.

Structure	Level	Pattern	Image	Note
TS22: submandibular gland primordium epithelium	Present		4B	Expression is in cell membranes. Strongest staining was seen in developing ducts, and weak staining was seen in end-pieces.
TS22: submandibular gland primordium mesenchyme	Ambiguous		4B	The authors state that staining on mesenchymal cells was equivocal.

Structure	Level	Pattern	Image	Note
TS23: submandibular gland primordium epithelium	Present		4C	Expression is in cell membranes. Strongest staining was seen in developing ducts, and weak staining was seen in end-pieces.
TS23: submandibular gland primordium mesenchyme	Ambiguous		4C	The authors state that staining on mesenchymal cells was equivocal.

Structure	Level	Pattern	Image	Note
TS24: submandibular gland epithelium	Present		4D	Expression is in cell membranes. Strongest staining was seen in developing ducts, and weak staining was seen in end-pieces.
TS24: submandibular gland mesenchyme	Ambiguous		4D	The authors state that staining on mesenchymal cells was equivocal.

Structure	Level	Pattern	Image	Note
TS25: submandibular gland epithelium	Present		4E	Expression is in cell membranes. Strongest staining was seen in developing ducts, and weak staining was seen in end-pieces.
TS25: submandibular gland mesenchyme	Ambiguous		4E	The authors state that staining on mesenchymal cells was equivocal.