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Gene Expression Data
Assay Details
Assay
Reference: J:106421 Komine Y, et al., Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci. 2006 Feb;31(2):273-83
Assay type: RNA in situ
MGI Accession ID: MGI:5632133
Gene symbol: Zfhx2
Gene name: zinc finger homeobox 2
Probe: zfh-5 cDNA Mix
Probe preparation: Antisense labelled with other - see notes RNA
Visualized with: Fluorescence
Assay notes: A mixed probe was used to improve sensitivity. The FITC-labeled probes were detected by a horseradish-peroxidase-conjugated anti-FITC antibody, amplified with a TSA-Plus DNP system and finally visualized by Alexa Fluor 488-conjugated anti-DNP antibody.
Results
Specimen 1C: embryonic day 14.5 (more )
Note: Double labeled: green - Zfhx2; red - Zfhx2os (assay MGI:5632134).
Structure Level Pattern Image Note
TS22: cerebral cortex Present 1C Sense , 1C Merge
TS22: cortical plate Present 1C Sense , 1C Merge
Specimen 1D: embryonic day 14.5 (more )
Note: Double labeled: green - Zfhx2; red - Zfhx2os (assay MGI:5632134).
Structure Level Pattern Image Note
TS22: forebrain Present 1D Merge , 1D Sense Expression is in the ventral forebrain.
Specimen 1E: embryonic day 14.5 (more )
Note: Double labeled: green - Zfhx2; red - Zfhx2os (assay MGI:5632134).
Structure Level Pattern Image Note
TS22: cerebral cortex Present 1E Sense , 1E Merge
TS22: thalamus Present 1E Sense , 1E Merge
Specimen 1F: embryonic day 14.5 (more )
Note: Double labeled: green - Zfhx2; red - Zfhx2os (assay MGI:5632134).
Structure Level Pattern Image Note
TS22: medulla oblongata Present 1F Merge , 1F Sense
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory