Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Pvalb-tdTomato)15Gfng
Name:	transgene insertion 15, Guoping Feng
MGI ID:	MGI:5629292
Transgene:	Tg(Pvalb-tdTomato)15Gfng  Location: unknown
Alliance:	Tg(Pvalb-tdTomato)15Gfng page

Strain of Origin:

C57BL/6NTac

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The mouse bacterial artificial chromosome (BAC) RP24-306A6 containing the entire Pvalb gene, was modified first by a BAC trimming method to remove the Ift27 gene coding region. The second BAC recombineering round inserted the tdTomato-WPRE-BGHpA (bovine growth hormone polyadenylation signal)-FRT-NEO-FRT vector directly downstream of the Pvalb initiation start codon. The FRT site flanked NEO was then removed by transient FLP recombinase expression. Correctly targeted BAC DNA was used as the source for the transgene, which was linearized to remove it from the vector. (J:101977, J:232250)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	21 reference(s)