Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:30430
Schonemann MD, et al., Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2. Genes Dev. 1995 Dec 15;9(24):3122-35
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5620384
|
|
Gene symbol:
|
Pou3f4
|
|
Gene name:
|
POU domain, class 3, transcription factor 4
|
|
Probe:
|
Brn-4 probe2
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
|
Results
|
Specimen
6A e13 upper left:
embryonic day 13.0
(more  )
Specimen
6A e13 upper left:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Clusters |
6A e13 upper left
|
Expressed in two clusters.
|
Specimen
6A e13 lower left:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Pou3f2tm1Rsd/Pou3f2tm1Rsd
|
|
Age:
|
embryonic day 13.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Clusters |
6A e13 lower left
|
Same as in wild-type.
|
Specimen
6A e16 upper left:
embryonic day 16.0
(more )
Specimen
6A e16 upper left:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hypothalamus |
Present |
Clusters |
6A e16 upper left
|
Expressed in two clusters. Decreased expression compared with that of E13.
|
Specimen
6A e16 lower left:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Pou3f2tm1Rsd/Pou3f2tm1Rsd
|
|
Age:
|
embryonic day 16.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hypothalamus |
Present |
Regionally restricted |
6A e16 lower left
|
Reduced expression in the dorsal portion of region I. Expression in region II is not substantially altered.
|
Specimen
6A e19 upper left:
embryonic day 19.0
(more )
Specimen
6A e19 upper left:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 19.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hypothalamus |
Present |
Clusters |
6A e19 upper left
|
Expressed in two clusters.
|
Specimen
6A e19 lower left:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Pou3f2tm1Rsd/Pou3f2tm1Rsd
|
|
Age:
|
embryonic day 19.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hypothalamus |
Present |
Regionally restricted |
6A e19 lower left
|
Only expressed in a few cells in region I. Expression in region II is not substantially altered.
|
Specimen
not shown:
embryonic day 10.0-11.0
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.0-11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon gland |
Present |
Regionally restricted |
|
Expressed in the primordium of the endocrine hypothalamus adjacent to the third ventricle.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Pou3f2tm1Rsd/Pou3f2tm1Rsd
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
|
|
Starting a progressive disappearance of positve cells in a dorsal to ventral fashion.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Pou3f2tm1Rsd/Pou3f2tm1Rsd
|
|
Age:
|
embryonic day 17.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
|
Most ventral expression in the paraventricular hypothalamic nucleus had been extinguished.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Pou3f2tm1Rsd/Pou3f2tm1Rsd
|
|
Age:
|
postnatal day 5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: paraventricular hypothalamic nucleus |
Present |
Regionally restricted |
|
Most ventral expression in the paraventricular hypothalamic nucleus had been extinguished. Only an occasional positive cell remained.
|
|
Analysis Tools