Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30430
Schonemann MD, et al., Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2. Genes Dev. 1995 Dec 15;9(24):3122-35
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5620379
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Gene symbol:
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Pou3f2
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Gene name:
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POU domain, class 3, transcription factor 2
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Antibody:
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anti-Brn-2
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
not shown:
embryonic day 13.0
(more  )
Note: Also stained for Pou3f4 (assay MGI:5620380).
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Also stained for Pou3f4 (assay MGI:5620380).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus |
Present |
Regionally restricted |
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Expressed in paraventricular hypothalamic nucleus.
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Specimen
not shown:
embryonic day 19.0
(more )
Note: Also stained for Pou3f4 (assay MGI:5620380).
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Also stained for Pou3f4 (assay MGI:5620380).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hypothalamus |
Present |
Regionally restricted |
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Expressed in paraventricular hypothalamic nucleus.
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Specimen
2C right:
postnatal
(more )
Note: Double labeled: red - Pou3f2; green - Pou3f4 (assay MGI:5620380).
Specimen
2C right:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pou3f2; green - Pou3f4 (assay MGI:5620380).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: supraoptic nucleus |
Present |
Regionally restricted |
2C right
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Neurons positive for only Pou3f2 are located predominantly on the dorsal periphery, whereas in ventral aspects are the Pou3f2 and Pou3f4 coexpressing neurons.
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Analysis Tools