Gene Expression Data
Assay Details
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Assay
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Reference:
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J:153112
Thesleff I, et al., Changes in the distribution of tenascin during tooth development. Development. 1987 Oct;101(2):289-96
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5619813
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Gene symbol:
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Tnc
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Gene name:
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tenascin C
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Antibody:
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Anti-tenascin
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Specimens were either frozen for cryosectioning or fixed in 94% ethanol and embedded in wax.
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Results
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Specimen
3A:
embryonic day 17.0
(more  )
Note: Bell stage.
Specimen
3A:
(close  )
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Genetic Background:
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(CBA x C57BL)F1
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Bell stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: dental papilla |
Strong |
Regionally restricted |
3A
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Only the intercuspal and cervical regions of the dental papilla were stained.
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| TS25: dental sac |
Absent |
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3A
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| TS25: jaw mesenchyme |
Strong |
Regionally restricted |
3A
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Expression was intense in the nondental mesenchyme surrounding the dental sac. This outermost mesenchyme represents osteogenic tissue associated with the ossifying mandibular bone.
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Specimen
3B:
embryonic day 17.0
(more )
Note: Bell stage. Section was digested with collagenase prior to staining.
Specimen
3B:
(close )
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Genetic Background:
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(CBA x C57BL)F1
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Note:
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Bell stage. Section was digested with collagenase prior to staining.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: dental papilla |
Strong |
Widespread |
3B
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After digestion with collagenase, positive staining became evident throughout the dental papilla.
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| TS25: dental sac |
Absent |
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3B
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| TS25: jaw mesenchyme |
Strong |
Regionally restricted |
3B
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Expression was intense in the nondental mesenchyme surrounding the dental sac. This outermost mesenchyme represents osteogenic tissue associated with the ossifying mandibular bone.
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| TS25: tooth basement membrane |
Present |
Regionally restricted |
3B
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After digestion with collagenase, expression was detected in the basement membrane under the enamel epithelium.
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