Antibody:	Anti-tenascin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Specimens were either frozen for cryosectioning or fixed in 94% ethanol and embedded in wax.

Structure	Level	Pattern	Image	Note
TS21: molar mesenchyme	Strong		1A
TS21: mandible mesenchyme	Present	Regionally restricted	1A	The osteogenic mesenchyme surrounding the forming mandibular bone shows staining.
TS21: jaw mesenchyme	Absent		1A	The loose mesenchyme of the jaw is negative.
TS21: Meckel's cartilage	Absent		1A

Structure	Level	Pattern	Image	Note
TS21: molar mesenchyme	Present	Regionally restricted	1B	Marked mesenchymal staining was present under the anterior aspect of the tooth bud.
TS21: mandible mesenchyme	Present	Regionally restricted	1B	The osteogenic mesenchyme surrounding the forming mandibular bone shows staining.
TS21: jaw mesenchyme	Absent		1B	The loose mesenchyme of the jaw is negative.
TS21: Meckel's cartilage	Absent		1B
TS21: tooth basement membrane	Present	Regionally restricted	1B	The basement membrane under the oral epithelium was positively stained particularly at the lingual side of the tooth bud.

Structure	Level	Pattern	Image	Note
TS22: tooth mesenchyme	Strong	Regionally restricted	2A	Expression was intense in the mesenchyme surrounding the epithelial cap, particularly in the region of the dental lamina connecting the dental to oral epithelium.
TS22: dental papilla	Present		2A	Staining in the dental papilla was less than in the surrounding mesenchyme.
TS22: tooth basement membrane	Present	Regionally restricted	2A	Expression was detected in the basement membranes.

Structure	Level	Pattern	Image	Note
TS22: tooth mesenchyme	Strong	Regionally restricted	2B	Expression was intense in the mesenchyme surrounding the epithelial cap, particularly in the region of the dental lamina connecting the dental to oral epithelium.
TS22: dental papilla	Present		2B	Staining in the dental papilla was less than in the surrounding mesenchyme.
TS22: tooth basement membrane	Present	Regionally restricted	2B	Expression was detected in the basement membranes. Treatment with hyaluronidase prior to staining increased the intensity.

Structure	Level	Pattern	Image	Note
TS23: tooth mesenchyme	Strong	Regionally restricted	2C	Expression was intense in the mesenchyme surrounding the tooth germ.
TS23: dental papilla	Present		2C	Staining in the dental papilla was less than in the surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: tooth mesenchyme	Strong	Regionally restricted	2D	Expression was intense in the mesenchyme surrounding the tooth germ.
TS23: dental papilla	Present		2D	Staining in the dental papilla was less than in the surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS27: molar dental papilla	Strong		4A , 4B
TS27: connective tissue	Present	Regionally restricted	4A	Expression was detected in the connective tissue overlying the tooth germs.
TS27: predentin	Absent		4B , 4A