Probe:	dystrophin probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	This probe detects the 14kb transcripts but not the 6.5 ones.

Structure	Level	Pattern	Image	Note
TS15: trunk myotome	Present	Widespread	1A/B	Expression is present throughout.

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Present		2A/B
TS22: diaphragm	Present		2A/B	Expression is present in the skeletal muscle.
TS22: cardiac muscle tissue	Present		2A/B
TS22: lung mesenchyme	Present		2A/B	Expression is present in the smooth muscle of the lung.
TS22: smooth muscle tissue	Present		2A/B	Expression is present in the smooth muscle of the lung.

Structure	Level	Pattern	Image	Note
TS23: axial musculature	Present		2C/D	Expression is present in trunk musculature.

Structure	Level	Pattern	Image	Note
TS25: hindlimb musculature	Present	Widespread	2E	Expression is present all muscles, including slow (eg the soleus) and fast (eg the extensor digitorum longus) ones.

Structure	Level	Pattern	Image	Note
TS14: cardiac muscle tissue	Trace

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Weak		3A/B

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		3C/D

Structure	Level	Pattern	Image	Note
TS19: heart atrium	Present	Widespread	4A/B
TS19: heart ventricle	Present	Widespread	4A/B

Structure	Level	Pattern	Image	Note
TS24: cardiac muscle tissue	Present

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present

Structure	Level	Pattern	Image	Note
TS20: lobar bronchus	Present	Regionally restricted	5A/B	Expression is present in the smooth muscle of the bronchi and bronchioli.
TS20: lung mesenchyme	Present	Regionally restricted	5A/B	Expression is present in the smooth muscle of the bronchi and bronchioli.
TS20: smooth muscle tissue	Present	Regionally restricted	5A/B	Expression is present in the smooth muscle of the bronchi and bronchioli.

Structure	Level	Pattern	Image	Note
TS23: blood vessel smooth muscle	Present		5C/D
TS23: skeletal muscle	Present		5C/D

Structure	Level	Pattern	Image	Note
TS22: esophagus mesenchyme	Present	Regionally restricted		Expression is present in the smooth muscle.
TS22: stomach mesenchyme	Trace	Regionally restricted		Expression is present in the smooth muscle.
TS22: intestine smooth muscle	Trace
TS22: spinal cord	Weak	Regionally restricted		Expression is present in a population of cells.

Structure	Level	Pattern	Image	Note
TS19: dorsal aorta	Present	Regionally restricted		Expression is present in smooth muscle.

Structure	Level	Pattern	Image	Note
TS20: extraembryonic vascular system	Present	Regionally restricted		Expression is present in smooth muscle.

Structure	Level	Pattern	Image	Note
TS23: intestine muscularis	Present		5E/F	Expression is present in smooth muscle.
TS23: stomach mesenchyme	Present		5E/F	Expression is present in smooth muscle.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Weak		6A/B
TS21: metencephalon	Weak		6A/B
TS21: pons	Weak		6A/B

Structure	Level	Pattern	Image	Note
TS21: epithalamus	Present		6C/D

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Strong	Regionally restricted	6E/F	Expression is present in the epiphysis (pineal body).

Structure	Level	Pattern	Image	Note
TS21: hypothalamus	Weak		7A/B
TS21: head	Present	Regionally restricted	7A/B	Expression is seen in tissue underlying the olfactory epithelium, on either side of the palate.

Structure	Level	Pattern	Image	Note
TS21: thalamus	Weak			Expression is present in a central region of the diencephalon.

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis	Strong		7C/D	Expression is present in Rathke's pocket.

Structure	Level	Pattern	Image	Note
TS21: spinal ganglion	Weak