Antibody:	anti-uvomorulin/E-Cadherin (DECMA-1), rat
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Strong		1a
TS19: pancreas primordium	Strong	Regionally restricted	1a	Expression is at the apical regions.
TS19: mesonephric tubule	Present	Regionally restricted	1a	Expression is in epithelial cells.
TS19: mesonephros	Present	Regionally restricted	1a	Expression is in epithelial cells of the mesonephric duct.
TS19: gonadal mesenchyme	Weak	Regionally restricted	1a	Staining is at areas where their membranes are contiguous with those of germ cells.

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present	Punctate	1b
TS19: mesonephric tubule	Present	Regionally restricted	1b	Expression is in epithelial cells.
TS19: mesonephros	Present	Regionally restricted	1b	Expression is in epithelial cells of the mesonephric duct.
TS19: gonadal mesenchyme	Weak	Regionally restricted	1b	Staining is at areas where their membranes are contiguous with those of germ cells.

Structure	Level	Pattern	Image	Note
TS19: mesonephric tubule	Present	Regionally restricted	1c	Expression in tubules closest to the gonadal primordium was deficient ventrally and showed reduced staining of their most ventral cells, especially at their apical ends.

Structure	Level	Pattern	Image	Note
TS20: mesonephric tubule	Present		1d
TS20: primordial germ cell of gonad	Present	Regionally restricted	1d	Expression is where the membranes of the cells are contiguous with each other.
TS20: gonadal mesenchyme	Weak		1d

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	2a	Expression is associated with cell clusters in the cortical region.

Structure	Level	Pattern	Image	Note
TS21: ovary	Present	Regionally restricted	2b	Expression is localized between germ cells.
TS21: mesenchymal stroma of ovary	Weak		2b
TS21: mesonephric tubule of female	Strong	Regionally restricted	2b	Expression is in epithelial cells.
TS21: paramesonephric duct of female, mesonephric portion	Strong	Regionally restricted	2b	Expression is in epithelial cells.

Structure	Level	Pattern	Image	Note
TS21: testis	Present	Regionally restricted	2c	Expression is in cell clusters in a cord-like arrangement, absent in tunica albuginea.

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present	Regionally restricted	2d	Expression is in cell clusters at areas of contact between adjacent cells.
TS21: testis mesenchyme	Present	Regionally restricted	2d	Expression is where somatic membranes are contiguous with those of germ cells.

Structure	Level	Pattern	Image	Note
TS22: germ cell of ovary	Strong	Regionally restricted	3a	Expression is between adjacent germ cells, weaker staining between germ cells and adjacent somatic cells.
TS22: mesenchymal stroma of ovary	Present	Regionally restricted	3a	Expression is between germ cells and adjacent somatic cells.

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present	Regionally restricted	3b	Expression is at the periphery of the cords.
TS22: germ cell of testis	Weak	Regionally restricted	3b
TS22: testis	Present	Regionally restricted	3b	Expression is at the periphery of the cords and is absent in the tunica albuginea.
TS22: mesonephric tubule of male	Present	Regionally restricted	3b	Expression is in epithelial cells.
TS22: nephric duct of male, mesonephric portion	Present	Regionally restricted	3b	Expression is in epithelial cells.

Structure	Level	Pattern	Image	Note
TS24: mesonephros	Strong	Regionally restricted		Expression is in the epithelial cells of the tubules.
TS24: ovary	Weak			Realtively even distribution. There is expression between germ cells and somatic cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: testis		Weak	Regionally restricted		Expression is at the basal membrane of cells that could be either germ cells or somatic cells.
TS24: Sertoli cell	Sertoli cell	Strong
TS24: interstitium of the testis		Weak	Regionally restricted		Expression was weak or absent between adjacent T-prospermatogonia.

Structure	Level	Pattern	Image	Note
TS25: ovary	Very strong	Regionally restricted	3c	Expression is at follicle-like cell aggregrates.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: Leydig cell	Leydig cell	Strong		3d
TS25: seminiferous cord		Strong	Regionally restricted	3d	Expression is in the region of basil lamina between Sertoli cells and peritubular myod cells. Weaker punctate staining between adjacent spermatogonia.

Structure	Level	Pattern	Image	Note
TS26: ovary	Weak	Diffuse

Structure	Cell Type	Level	Pattern	Image	Note
TS26: Leydig cell	Leydig cell	Strong	Regionally restricted		Expression is in the areas of contacts.