Antibody:	Anti-TGF-beta 2
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Present	Regionally restricted	1A	Intense immunostaining was evident in the oral epithelium.
TS19: oral region mesenchyme	Present	Regionally restricted	1A	Diffuse expression was detected in the mesenchyme surrounding the thickening epithelium as it forms the dental lamina.

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Present	Regionally restricted	1B	Intense immunostaining was evident in the oral epithelium, but the basement membrane and epithelial cells in the basal layer showed no immunostaining.
TS19: oral region mesenchyme	Present	Regionally restricted	1B	Diffuse expression was detected in the mesenchyme surrounding the thickening epithelium as it forms the dental lamina.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	1C	Intense immunostaining was evident in the oral epithelium.
TS20: oral region mesenchyme	Present	Regionally restricted	1C	Diffuse expression was detected in the mesenchyme surrounding the thickening epithelium as it forms the dental lamina.

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Present	Regionally restricted	1D	Intense immunostaining was evident in the oral epithelium, but the basement membrane and epithelial cells in the basal layer showed no immunostaining.
TS20: oral region mesenchyme	Present	Regionally restricted	1D	Diffuse expression was detected in the mesenchyme surrounding the thickening epithelium as it forms the dental lamina.

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar epithelium	Present	Regionally restricted	1E	Expression was detected in the dental epithelium, but absent in the basement membrane and epithelial cells in the basal layer. At the tip of the dental epithelium, the staining was pronounced.
TS21: lower jaw molar mesenchyme	Present	Regionally restricted	1E	Expression was detected in the mesenchyme surrounding the dental epithelium.

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar epithelium	Present	Regionally restricted	1F	Expression was detected in the dental epithelium, but absent in epithelial cells in the basal layer.
TS21: lower jaw molar mesenchyme	Present	Regionally restricted	1F	Expression was detected in the mesenchyme surrounding the dental epithelium.
TS21: tooth basement membrane	Absent		1F

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar epithelium	Present	Regionally restricted	2A	Expression was abundant in the outer dental epithelium and inner dental epithelium.
TS22: enamel knot	Present		2A
TS22: lower jaw molar mesenchyme	Weak		2A
TS22: stellate reticulum	Weak		2A

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar epithelium	Present	Regionally restricted	2B	Expression was abundant in the outer dental epithelium and inner dental epithelium.
TS22: enamel knot	Present	Regionally restricted	2B	Expression was detected in the primary enamel knot adjacent to and away from the basement membrane.
TS22: tooth basement membrane	Absent		2B

Structure	Level	Pattern	Image	Note
TS23: lower jaw molar mesenchyme	Present	Regionally restricted		Expression was detected in the dental mesenchymal cells adjacent to the inner dental epithelium.
TS23: lower jaw molar epithelium	Present	Regionally restricted		Expression was detected in the inner and outer dental epithelium.
TS23: cervical loop	Present

Structure	Level	Pattern	Image	Note
TS24: lower jaw molar epithelium	Strong	Regionally restricted	2C	Strong staining was detected in the inner dental epithelium. Weak staining was detected in the outer dental epithelium.
TS24: enamel knot	Strong	Regionally restricted	2C	Strong expression was detected in the secondary enamel knot.
TS24: cervical loop	Strong		2C
TS24: lower jaw molar mesenchyme	Strong	Regionally restricted	2C	Strong expression was detected in the cervical loop and adjacent dental mesenchymal cells.
TS24: stellate reticulum	Weak		2C

Structure	Level	Pattern	Image	Note
TS24: enamel knot	Present	Regionally restricted	2D	Expression was detected in the secondary enamel knot. Only cells away from the basement membrane showed staining.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar	Present	Regionally restricted	3A	Expression was intense in odontoblasts. Expression was weak in preodontoblasts and preameloblasts.
TS26: lower jaw molar dental papilla	Present	Regionally restricted	3A	Expression was detected in dental papilla adjacent to dental cusp.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar	Present	Regionally restricted	3B	Expression was intense in odontoblasts and weak in preameloblasts.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Strong	Regionally restricted	3C	Expression was strong in odontoblasts. Immunostaining in ameloblasts gradually increased.
TS27: lower jaw molar dental papilla	Strong	Regionally restricted	3C	Expression was detected in ectomesenchymal cells in the coronal part of the dental papilla.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Strong	Regionally restricted	3D	Expression was strong in odontoblasts. Immunostaining in ameloblasts gradually increased.
TS27: lower jaw molar dental papilla	Strong	Regionally restricted	3D	Expression was detected in ectomesenchymal cells in the coronal part of the dental papilla.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Strong	Regionally restricted	3E	Expression was strong in odontoblasts. Immunostaining in ameloblasts was more pronounced.
TS27: lower jaw molar dental papilla	Strong	Regionally restricted	3E	Expression was detected in the dental papilla adjacent to the dental cusp.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Strong	Regionally restricted	3F	Expression was strong in odontoblasts. Immunostaining in ameloblasts was more pronounced.
TS27: lower jaw molar dental papilla	Strong	Regionally restricted	3F	Expression was detected in the dental papilla adjacent to the dental cusp.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Present	Regionally restricted	4A	Odontoblasts remained immunopositive. Mature ameloblasts lost immunoreactivity. Secretory ameloblasts adjacent to the dental cusp exhibit positive staining.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Present	Regionally restricted	4B	Odontoblasts remained immunopositive. Mature ameloblasts lost immunoreactivity.

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar	Present	Regionally restricted	4C	Odontoblasts remained immunopositive. Secretory ameloblasts adjacent to the dental cusp exhibit positive staining.