Antibody:	Anti-Fgr
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present
TS13: neural tube	Present
TS13: neural ectoderm	Present	Regionally restricted		Expression was detected in the neural tube when it was partially closed.

Structure	Level	Pattern	Image	Note
TS17: brain	Strong
TS17: future spinal cord	Strong
TS17: forebrain	Present	Regionally restricted		Expression was dorsal.
TS17: midbrain	Present	Regionally restricted		Expression was dorsal.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted		Expression was dorsal.
TS19: midbrain	Present	Regionally restricted		Expression was dorsal.

Structure	Level	Pattern	Image	Note
TS20: forebrain ventricular layer	Weak
TS20: midbrain roof plate	Weak
TS20: midbrain ventricular layer	Weak
TS20: forebrain	Weak	Regionally restricted		Expression was low in the roof of the forebrain.
TS20: spinal nerve	Present

Structure	Level	Pattern	Image	Note
TS20: vagus ganglion	Present		2D
TS20: vagal nerve trunk	Present		2D	Expression was noted around the heart and respiratory tract.